Applications:
Separation of Sulphonium Ions in Polar Organic Mode
The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both polar organic and ion-exchange modes in the separation of sulfonium ions. The high organic mobile phase retains methane sulfonic acid by a polar organic mechanism and separates methoxydimethylsulphonium and trimethylsulfphonium cations by polar organic and cation exchange modes. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN), and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD).
| Column | Primesep 100, 4.6x250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 35/65% |
| Buffer | TFA |
| Flow Rate | 1.5 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sulphonium Ions, Methoxydimethylsulphonium, Trimethylsulfphonium |
Application Analytes:
Methanesulfonic Acid
Methoxydimethylsulphonium
Sulfonium Ions
Trimethylsulfonium