|Molecular Weight||58.693 g/mol|
Primesep 100 allows the quantitation of nickel cation in a hydrogenation product. Nickel is retained by cation exchange and the hydrogenation product is retained by hydrophobic, reversed-phase mechanisms. Adding an ELSD detector to the system allows detection of nickel which was not possible with UV alone. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD) and UV detection.
|Column||Newcrom A, 4.6x150 mm, 5 µm, 100A|
|Mobile Phase||MeCN - 10%|
|Buffer||Gradient Ammonium formate pH 3.0|
|Flow Rate||1.0 ml/min|
|Class of Compounds||Hydrophilic, Metal, Ion|
|Analyzing Compounds||Sodium, Potassium, Copper, Nickel, Barium, Neodymium, Gadolinium|