Nicotinamide Adenine Dinucleotide (NAD)

CAS Number 53-84-9
Molecular Formula C21H27N7O14P2
Molecular Weight 663.430 g/mol
LogP -4.17
  • Nadide
  • 53-84-9
  • 5-26-16-00399
  • Adenosine 5'-(trihydrogen diphosphate), P'-5'-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium, inner salt
  • Adenine-nicotinamide dinucleotide
  • Adenosine 5'-(trihydrogen diphosphate), 5'-5'-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium hydroxide, inner salt
  • Adenosine 5'-(trihydrogen diphosphate), P'-5'-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium hydroxide, inner salt
  • Codehydrase I
  • Codehydrogenase I
  • Coenzyme I
  • Cozymase I
  • Diphosphopyridine nucleotide
  • Enzopride
  • Nicotinamide adenine dinucleotide
  • Nicotinamide-adenine dinucleotide
  • NSC 20272
  • Oxidized diphosphopyridine nucleotide
  • Pyridinium, 3-carbamoyl-1-β-D-ribofuranosyl-, hydroxide, 5'-5'-ester with adenosine 5'-(trihydrogen pyrophosphate), inner salt
  • β-Diphosphopyridine nucleotide
  • β-NAD+
  • β-Nicotinamide adenine dinucleotide
  • β-Nicotineamide adenine dinucleotide
  • BRN 3584133
  • EINECS 200-184-4
  • Nicotinamide dinucleotide
  • Nicotineamide adenine dinucleotide
  • Pyridine, nucleotide diphosphate
  • beta-Diphosphopyridine nucleotide
  • beta-NAD
  • beta-NAD+
  • beta-Nicotinamide adenine dinucleotide
  • Nadidum
  • UNII-0U46U6E8UK
  • 1083039-67-1
  • 1354693-98-3
  • 159929-29-0
  • 30429-30-2


Separation of Nicotinamide and Related Substances

A complex mixture of nicotinamide and related impurities was separated on Obelisc R mixed-mode column. Nicotinamide, methylnicotinamide, nicotinamide adenine mononucleotide, nicotinamide adenine dinucleotide, and adenosine monophosphate were baseline resolved in a 15 minute long method. This mixed-mode approach can be used for analysis of other nucleotides. Obelisc R trimodal column separates this complex mixture based on reversed-phase, cation-exchange and anion-exchange mechanisms. Retention is controlled by amount of ACN, buffer concentration and buffer pH. Additional selectivity can be gained by exploring various buffers within the same pH

Application Analytes:

Adenosine Monophosphate
Nicotinamide Adenine Dinucleotide (NAD)
Nicotinamide Adenine Mononucleotide

HPLC Separation of NAD and NADH on PEI Column
Separation type: Liquid Chromatography Mixed-mode View on

High Performance Liquid Chromatography (HPLC) Method for Analysis of SNAD and NADH

Nicotinamide adenine dinucleotide (NAD), is a coenzyme found in every single living cell. NAD can exist in two forms: NAD+ and NADH. The conversion of NAD from its oxidized form (NAD+) to its reduced form (NADH), and back, provides the cell with a mechanism for accepting and donating electrons. NAD and NADH can be retained, separated and UV detected at 260 nm using the PEI column with a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer and detected by UV, ELSD, CAD or LC/MS.


Column PEI, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Ammonium Acetate pH 6.8 - 60 mM
Flow Rate 2.0 ml/min
Detection UV 260 nm


Class of Compounds  Drug
Analyzing Compounds NAD, NADH

Application Analytes:

Nicotinamide Adenine Dinucleotide (NAD)
Nicotinamide Adenine Dinucleotide (reduced) (NADH)