Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

Nicotinic Acid

CAS Number 59-67-6
Molecular Formula C6H5NO2
Molecular Weight 123.111 g/mol
InChI Key PVNIIMVLHYAWGP-UHFFFAOYSA-N
LogP 0.4
Synonyms
  • niacin
  • Pyridine-3-carboxylic acid
  • 3-pyridinecarboxylic acid
  • 3-Carboxypyridine
  • wampocap
  • vitamin B3
  • Acidum nicotinicum
  • Apelagrin
  • Pellagrin
  • Akotin
  • Daskil
  • Efacin
  • Pelonin
  • Linic
  • nicamin
  • nicobid
  • nicocap
  • nicolar
  • Enduracin
  • Nicodelmine
  • Niconacid
  • Nicotinipca
  • Pellagramin
  • Direktan
  • Niaspan
  • Nicacid
  • Nicangin
  • Peviton
  • Bionic
  • Diacin
  • Nicyl
  • Nyclin
  • Niac
  • Vitaplex N
  • Davitamon PP
  • Nico-Span
  • Tega-Span
  • Nicocidin
  • Nicocrisina
  • Niconazid
  • Nicoside
  • Nicotamin
  • Nicotene
  • Nicovasan
  • Nicovasen
  • Nipellen
  • SK-Niacin
  • Naotin
  • Nicodan
  • Nicodon
  • Niconat
  • Nicosan 3
  • Nicosyl
  • Nicotil
  • Tinic
  • 3-Carboxylpyridine
  • Nicotine acid
  • Niacor

Applications:


HPLC Separation of Carboxylic Acids
HPLC.cloud View on hplc.cloud

Condition 1

Column Newcrom  B, 3.2x50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O- 30/70%
Buffer H3PO4 - 0.5%
Flow Rate 0.5 ml/min
Detection UV, 275 nm
 
HPLC separation of carboxylic acids (nicotinic acid, benzoic acid and acetylbenzoic acid) on Primesep B2 column using an isocratic method by reverse-phase and anion-exchange mechanisms. The elution of carboxylic acids using the same isocratic method and mobile phase can be reversed by using a reverse-phase cation-exchange mechanisms of the Primesep 100 column. The mobile phase is water, acetonitrile (MeCN, ACN) and formic acid with a UV detector.

Condition 2

Column Primesep B2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds Nicotinic acid, Benzoic acid, Acetylbenzoic acid, 1.4-acetylbenzoic Acid
 

Application Analytes:

Acetylbenzoic Acid
Benzoic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

HPLC Separation of Pyridinecarboxylic Acids
Pyridinecarboxylic acids exist as three isomers with different position of carboxylic acid relative to nitrogen in pyridine. Three isomers of pyridinecarboxylic acid (picolinic or 2-pyridinecarvoxylic acid, niacin or 3-pyridinecarboxylic acid, isonicotinic or 4-pyridinecarboxylic acid), along with pyridinedicarboxylic acid, are separated on a Primesep 100 column. Pyridinecarboxylic acids have a similar empirical formula, and are very similar in terms of hydrophobicity and ionic properties. Small differences in these properties are enough to achieve good separation on cation-exchange mixed-mode HPLC column like Primesep 100. Retention time for all compounds is controlled by the amount of acetonitrile and amount of ions in the mobile phase. Ions in the mobile phase can be created by organic and inorganic acids and corresponding salt buffers. Various detection techniques can be used for monitoring pyridinecarboxylic acids. Other ionizable isomers can be successfully separated on mixed-mode columns.

Application Analytes:

Isonicotinic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Organic Acids
Picolinic Acid
Pyridinedicarboxylic Acid

HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities
Vitamin C (ascorbic acid) and Vitamins Group B are separated on Obelisc N mixed-mode column. Method can be used in quantitation and determination of polar vitamins in various formulations and dietary supplements. HPLC method can be based on UV, Evaporative Light Scattering Detection (ESLD), RI or MS detection. Effect of sample matrix can be eliminated by changing mobile phase conditions. Buffer concentration, buffer pH and amount of ACN will affect every vitamin differently due to difference in polar and ionic properties.

Condition

Column Obelisc N , 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 5.0
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine, Ascorbic acid, Niacinamide, Pantothenic acid, Caffeine, Riboflavin, Barbituric Acid,  3- Aminopyrine


Application Analytes:

3-Aminopyridine
4-Aminopyridine/Fampridine
Ascorbic Acid
Barbituric Acid
Isonicotinic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pyridoxine
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)

HILIC Separation of Vitamins Group B
Separation of vitamins group B is achieved on Obelisc N column in HILIC mixed-mode. Vitamins of this group are different in polarity and ionic properties. Retention and separation is achieved by optimization of amount of ACN, buffer and buffer pH. Combination of UV and ELSD detection is used to monitor HPLC separation. B vitamins are water-soluble vitamins that play an important role in cell metabolism. Supplements containing all six are generally referred to as a vitamin B complex. Individual B vitamin supplements are referred to by the specific name of each vitamin. This method can be used to analyze individual B vitamins as well as vitamin B complex. Isolation of impurities as well as degradation products is possible by preparative chromatography.

Condition

Column Obelisc N , 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAC pH 4.5
Flow Rate 1.0 ml/min
Detection UV, 270 nm, ELSD
 

Description

Class of Compounds Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine,  Niacinamide, Pantothenic acid, Riboflavin


Application Analytes:

Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B3 (Niacin)
Vitamin B5 (Pantothenic Acid)
Vitamin B6 (Pyridoxine)

Analysis of Nicotinic Acid and Nicotinamide (Vitamin B3) on Newcrom AH Column
HPLC.cloud View on hplc.cloud Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH  was used to separate nicotinamide and nicotinic acid.  Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.

Condition

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer H3PO4 - 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds  Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Nicotinic Acid, Nicotinamide, Vitamin B3
 

Application Analytes:

Niacin
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

HPLC Separation Of Mixture of Pyridylacetic Acids And Their Derivatives


Application Analytes:

2-Pyridylacetic acid hydrochloride
3-Acetylpyridine
3-Pyridylacetic acid hydrochloride
4-Pyridylacetic acid hydrochloride
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pyridyl-3-thioacetomorpholide