Nicotinic Acid

CAS Number	59-67-6
Molecular Formula	C6H5NO2
Molecular Weight	123.111 g/mol
InChI Key	PVNIIMVLHYAWGP-UHFFFAOYSA-N
LogP	0.4
Synonyms	niacin Pyridine-3-carboxylic acid 3-pyridinecarboxylic acid 3-Carboxypyridine wampocap vitamin B3 Acidum nicotinicum Apelagrin Pellagrin Akotin Daskil Efacin Pelonin Linic nicamin nicobid nicocap nicolar Enduracin Nicodelmine Niconacid Nicotinipca Pellagramin Direktan Niaspan Nicacid Nicangin Peviton Bionic Diacin Nicyl Nyclin Niac Vitaplex N Davitamon PP Nico-Span Tega-Span Nicocidin Nicocrisina Niconazid Nicoside Nicotamin Nicotene Nicovasan Nicovasen Nipellen SK-Niacin Naotin Nicodan Nicodon Niconat Nicosan 3 Nicosyl Nicotil Tinic 3-Carboxylpyridine Nicotine acid Niacor

Applications:

HPLC Separation of Carboxylic Acids

HPLC separation of carboxylic acids (nicotinic acid, benzoic acid and acetylbenzoic acid) on Primesep B2 column using an isocratic method by reverse-phase and anion-exchange mechanisms. The elution of carboxylic acids using the same isocratic method and mobile phase can be reversed by using a reverse-phase cation-exchange mechanisms of the Primesep 100 column. The mobile phase is water, acetonitrile (MeCN, ACN) and formic acid with a UV detector.

Acetylbenzoic Acid
Benzoic Acid
Nicotinic Acid

HPLC Separation of Pyridinecarboxylic Acids

Pyridinecarboxylic acids exist as three isomers with different position of carboxylic acid relative to nitrogen in pyridine. Three isomers of pyridinecarboxylic acid (picolinic or 2-pyridinecarvoxylic acid, niacin or 3-pyridinecarboxylic acid, isonicotinic or 4-pyridinecarboxylic acid), along with pyridinedicarboxylic acid, are separated on a Primesep 100 column. Pyridinecarboxylic acids have a similar empirical formula, and are very similar in terms of hydrophobicity and ionic properties. Small differences in these properties are enough to achieve good separation on cation-exchange mixed-mode HPLC column like Primesep 100. Retention time for all compounds is controlled by the amount of acetonitrile and amount of ions in the mobile phase. Ions in the mobile phase can be created by organic and inorganic acids and corresponding salt buffers. Various detection techniques can be used for monitoring pyridinecarboxylic acids. Other ionizable isomers can be successfully separated on mixed-mode columns.

Isonicotinic Acid
Nicotinic Acid
Organic Acids
Picolinic Acid
Pyridinedicarboxylic Acid

HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities

Vitamin C (ascorbic acid) and Vitamins Group B are separated on Obelisc N mixed-mode column. Method can be used in quantitation and determination of polar vitamins in various formulations and dietary supplements. HPLC method can be based on UV, Evaporative Light Scattering Detection (ESLD), RI or MS detection. Effect of sample matrix can be eliminated by changing mobile phase conditions. Buffer concentration, buffer pH and amount of ACN will affect every vitamin differently due to difference in polar and ionic properties.

3-Aminopyridine
4-Aminopyridine/Fampridine
Ascorbic Acid
Barbituric Acid
Isonicotinic Acid
Nicotinic Acid
Pyridoxine
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)

HILIC Separation of Vitamins Group B

Separation of vitamins group B is achieved on Obelisc N column in HILIC mixed-mode. Vitamins of this group are different in polarity and ionic properties. Retention and separation is achieved by optimization of amount of ACN, buffer and buffer pH. Combination of UV and ELSD detection is used to monitor HPLC separation. B vitamins are water-soluble vitamins that play an important role in cell metabolism. Supplements containing all six are generally referred to as a vitamin B complex. Individual B vitamin supplements are referred to by the specific name of each vitamin. This method can be used to analyze individual B vitamins as well as vitamin B complex. Isolation of impurities as well as degradation products is possible by preparative chromatography.

Nicotinic Acid
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B3 (Niacin)
Vitamin B5 (Pantothenic Acid)
Vitamin B6 (Pyridoxine)

Analysis of Nicotinic Acid in Nicotinamide

Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Primesep 100 was used to separate nicotinamide and nicotinic acid. Concentrations of nicotinic acid as low as 0.1ppm could be used.Primesep 100 separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.

Condition
Column	Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase	MeCN/H2O - 5/95%
Buffer	Na2HPO4 pH 3.0- 10 mM
Flow Rate	1.0 ml/min
Detection	UV, 230 nm