Nile Blue A

CAS Number 3625-57-8
Molecular Formula C40H40N6O6S
Molecular Weight 732.856 g/mol
InChI Key QIRDPEPUXNCOLD-UHFFFAOYSA-N
Synonyms
  • Nile Blue sulfate
  • Nile Blue A
  • Nile Blue A sulfate
  • 3625-57-8
  • Nile blue
  • NSC 3250
  • EINECS 222-832-5
  • NSC 34914
  • MFCD00064529
  • SBB057664

Applications:


HPLC Dye Separation on SHARC1 Column
 

Condition

Column Sharc 1, 3.2x100 mm, 3 µm, 100A
Mobile Phase MeCN/EtOH - 99/1%
Buffer H2SO4 - 0.5%
Flow Rate 1.5 ml/min
Detection UV, 500, 650 nm
 

Description

Class of Compounds Dye, Hydrophilic, Ionizable
Analyzing Compounds Methyl Red, Methyl Orange, Methylene Blue, Nile Blue A
 

Application Analytes:

Methyl Orange
Methyl Red
Methylene Blue
Nile Blue A

HPLC Method for Analysis of Dyes
HPLC Separation of Dyes

3-Aminoacridine is an aminoacridine. Alizarin a red pigment that comes as a red powder with a slight orange tint. When dissolved it appears bright red with a slight pink undertone. Alizarin is also known as Mordant Red 11 or Turkey Red. Eosin Y is a pigment that comes as an orange powder that when dissolved in water turns bright orange. Eosin is a fluorescent staining agent. It is used to stain red blood cells, proteins on cytoplasm, collage, and muscle fibers. It is also fluorescence. Fluorescein is a dye that comes in a dark orange powder and is used as a tracer and an indicator. As it says in the name, it is also Fluorescent, obtaining a green-yellow color when under black light. Methyl red an indicator that turns red in acidic solutions (below 4.4 pH), is a shade of orange between 4.5 and 6.1 pH, and turns bright yellow in basic solutions (above pH 6.2). Nile blue, also known as Nile blue A, is used as a stain used in biology and histology as well as a pH indicator. In pH below 7 it becomes lighter shades of glue, and at pH 0 becomes almost completely translucent. At pH of seven it has a dark blue shade, while at pH higher than that it starts becoming more red. Pararosaniline Hydrochloride, also known as Basic Red 9, or C.I. 42500. It is a a magenta solid that is used as a dye. Patent Blue Violet, also known as Patent Blue, is a deep blue pigment that is often used to dye clothes. Resazurin is a blue dye as well as a pH indicator. At pH 3.8 or lower, it is bright orange, but the higher the pH the more blue it becomes, the color stops changing at the pH of 6.5 where it becomes indigo. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain dyes. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Analytes:

3-Aminoacridine
Alizarin
Carmine
Eosin Y
Fluorescein
Methyl Red
Nile Blue A
Pararosaniline Hydrochloride
Patent Blue Violet (Patent Blue)
Resazurin