| CAS Number | 14797-55-8 |
|---|---|
| Molecular Formula | NO3 |
| Molecular Weight | 62.005 g/mol |
| InChI Key | NHNBFGGVMKEFGY-UHFFFAOYSA-N |
| LogP | 0.21 |
| Synonyms |
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Applications:
HPLC Separation of Chloride and Nitrate Ions
High nitrate concentrations in water and soil can be toxic to humans, fish and domestic animals. Chloride can be used for analysis of water contamination. Primesep D column can be used to separate and quantify both ions. The baseline separation between chloride and nitrate can be increased by decreasing the ammonium formate concentration in the mobile phase. Evaporative Light Scattering Detection (ELSD) used.
| Column | Primesep D, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Hydrophilic, Ion |
| Analyzing Compounds | Chloride, Nitrate |
Application Analytes:
Chloride
Nitrate
Nitric Acid
Sodium
HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Obelisc N
Ion chromatography is usually used for analysis of hydrophilic organic and inorganic ions. Same separation can be achieved on HILIC/mixed-mode Obelisc N HPLC columns. Obelisc N HPLC columns have very polar groups on their surface: one of the groups is basic and the other acidic. In case of low organic concentration, two groups are connected by hydrophilic linker. Obelisc N column can be used as cation-exchange and anion-exchange column. This allows to separate positively and negatively charged molecules in one run. Five anions (chloride, bromide, methanesulfonate, nitrate and perchlorate) along with one cation (sodium) were separated in one run. Method is compatible with ELSD, CAD and LC/MS and can be used for analysis of various hydrophilic and hydrophobic cations and anions in one HPLC run.
| Column | Obelisc N, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Hydrophilic, Ions |
| Analyzing Compounds | Chloride, Nitrate, Chlorate, Bromide, Potassium |
Application Analytes:
Bromide
Chloride
Methanesulfonic Acid
Nitrate
Nitric Acid
Potassium
HPLC Separation of Inorganic Anions
Organic and inorganic acids and ions can be separated on a Primesep B4 column based on their ionic properties. Method can be used for quantitation of residual acids in various products and sample matrices. Trifluoracetic, hydrochloric, methanesulfonic, and nitric acids are separated using ACN-water-ammonium formate. Ions can be detected by ELSD, CAD or LC/MS.
| Column | Primesep B4, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Phosphate, Chloride, Nitrate, Sulfate, Iodide, Perchlorate, Trifluoracetic |
Application Analytes:
Hydrochloric Acid
Methanesulfonic Acid
Nitrate
Nitric Acid
Organic Acids
Perchloric Acid
TFA (Trifluoroacetic Acid)
HPLC Separation of Inorganic Anions on Newcrom B Column
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| Column | Newcrom B, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 10/90% |
| Buffer | Gradient AmAc - 20-50 mM , 11 min |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Phosphate, Chloride,Bromide, Nitrate, Sulfate, Iodide, Perchlorate |
Application Analytes:
Bromide
Chloride
Iodine
Nitrate
Perchlorate
Phosphate
Sodium
Sulfate
HPLC Separation of Inorganic Anions on Newcrom BH Column
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| Column | Newcrom BH, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 10/90% |
| Buffer | Gradient AmAc pH 5.0 - 20-90 mM , 20 min |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Phosphate, Chloride, Bromide, Nitrate, Iodide, Sulfate, Perchlorate |
Application Analytes:
Bromide
Chloride
Iodine
Nitrate
Perchlorate
Phosphate
Sodium
Sulfate