Phosphate

CAS Number 14265-44-2
Molecular Formula O4P
Molecular Weight 94.971 g/mol
InChI Key NBIIXXVUZAFLBC-UHFFFAOYSA-K
LogP -0.770
Synonyms
  • Phosphate
  • Phosphoric acid, ion(3-)
  • 14265-44-2
  • inorganic phosphate (Pi)
  • Orthophosphate
  • Orthophosphate (PO43-)
  • Orthophosphate(3-)
  • Phosphate (PO43-)
  • Phosphate anion(3-)
  • Phosphate ion (PO43-)
  • Phosphate ion(3-)
  • Phosphate trianion
  • Phosphate(3-)
  • Phosphoric acid, ion(3-)
  • o-Phosphate
  • Phosphate ions
  • UNII-NK08V8K8HR
  • NFB Orthophosphate
  • O-Phosphoric acid
  • Ortho-phosphate
  • PO4(3-)
  • Phosphate trianion
  • Phosphoric acid ion(3-)
  • Pi
  • [PO4](3-)
  • 264888-19-9

Applications:


HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC
Sulfate and phosphate are separated on Primesep B2 and Primesep D column by anion-exchange mechanism. Because both sulfate and phosphate ions are not UV-active, ELSD is used to monitor separation of both anions. Method can be used to quantitate phosphate and sulfate in various pharmaceutical and chemical formulation, products and solution, drinking and ground water. Retention time is adjusted by increase or decrease of buffer concentrations. Two anions can be retained and separated on a very short column due to strong ion-exchange interaction. Method shows good reproducibility and versatility.

Condition

Column Primesep B2, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Ions,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Sodium, Phosphate, Sulfate


Application Analytes:

Phosphate
Sodium
Sulfate

HPLC Analysis of Sulfate and Phosphate Ions on Primesep Columns


Inorganic and organic ions are usually analyzed by ion-exchange mechanism. In this application, we used short Primesep B2 and Primesep D mixed-mode HPLC columns to retain and separate phosphate and sulfate ions. Both ions are retained by ion-exchange mechanism. Detection technique for analysis of these anions is Evaporative Light-Scattering Detector (ELSD).



Application Analytes:

Phosphate
Sulfate

HPLC Separation of Citric Acid and Phosphate Ions in HILIC Chromatography



Citric acid and phosphate ion are separated by HILIC on Primesep N HILIC HPLC column. Both compounds are very hydrophilic. Citric acid and phosphate ions are well separated with excellent peak shape. Method can be used for quantitation of citrate and phosphate in various formulations. Because of the lack of UV activity, separation can be monitored by LC/MS, ELSD or Corona CAD. Method can be used for other hydrophilic organic and inorganic acids.



Application Analytes:

Citric Acid
Phosphate

HPLC Separation of Inorganic Anions on Newcrom B Column
HPLC.cloud View on hplc.cloud  

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer Gradient AmAc -  20-50 mM , 11 min
Flow Rate 1.0 ml/min
Detection CAD

Description

Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Phosphate, Chloride,Bromide, Nitrate, Sulfate, Iodide, Perchlorate


Application Analytes:

Bromide
Chloride
Iodine
Nitrate
Perchlorate
Phosphate
Sodium
Sulfate

HPLC Separation of Inorganic Anions on Newcrom BH Column
HPLC.cloud View on hplc.cloud  

Condition

Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer Gradient AmAc -  20-90 mM , 20 min
Flow Rate 1.0 ml/min
Detection CAD (Corona)  (MS-compatible mobile phase)

Description

Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Phosphate, Chloride, Bromide, Nitrate, Sulfate, Iodide, Perchlorate


Application Analytes:

Bromide
Chloride
Iodine
Nitrate
Perchlorate
Phosphate
Sodium
Sulfate

HPLC Separation of Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column
HPLC Separation of Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric acids on Newcrom B Column_1201  

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/78%
Buffer Formic Acid - 2%
Flow Rate 1.0 ml/min
Detection CAD

Description

Class of Compounds Acids, Hydrophilic, Ionizable
Analyzing Compounds Ethylphosphonic acid,                      Phosphoric, Methylphosphonic Acid, Methylenediphosphonic acid


Application Analytes:

Ethylphosphonic Acid
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate

HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column
HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column_1202    

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 20%
Buffer Formic Acid - 0.5%
Flow Rate 1.0 ml/min
Detection CAD
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Phosphate, Glyphosate


Application Analytes:

Glyphosate
Phosphate

HPLC Separation of Glyphosate, Ethylphosphonic Acid and Methylphosphonic Acid on Newcrom B Column
HPLC Separation of Glyphosate, Ethylphosphonic Acid and Methylphosphonic Acid on Newcrom B Column_1203

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 40/59%
Buffer Formic Acid - 1%
Flow Rate 1.0 ml/min
Detection CAD

Description

Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Ethylphosphonic acid,                      Phosphoric Acid, Methylphosphoric Acid, Glyphosate


Application Analytes:

Ethylphosphonic Acid
Glyphosate
Methylphosphonic Acid
Phosphate

HPLC Separation of Glyphosate, Glyphosine, Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column
 

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN Gradient  -40-10%, 15 min
Buffer Formic Acid Gradient  -1- 5%, 15 min
Flow Rate 1.0 ml/min
Detection CAD
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Description

Class of Compounds Acids, Plant growth regulator, Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Ethylphosphonic acid,                      Phosphate Ion, Methylphosphonate, Methylenediphosphonic acid, Glyphosate, Glyphosine


Application Analytes:

Ethylphosphonic Acid
Glyphosate
Glyphosine
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate