| CAS Number | 7664-38-2 |
|---|---|
| Molecular Formula | H3O4P |
| Molecular Weight | 97.994 g/mol |
| InChI Key | NBIIXXVUZAFLBC-UHFFFAOYSA-N |
| LogP | -0.770 |
| Synonyms |
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Applications:
HPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography
The majority of drugs in the pharmaceutical industry are administered in salt form. The presence of two counter-ions very often necessitates the use of two methods. The nature of these counterparts in drugs can be an inorganic cation and organic acid, inorganic anion and organic base, and organic cation and organic anion. Furthermore, the properties of the molecules will result in a differing stoichiometry. The task of simultaneous quantitation of counter-ions can be achieved by using mixed-mode columns. The general approach for analysis is based on properties of corresponding counter-ions. Hydrophobic basic drugs, like dextromethorphan, verapamil, trimipramine, and corresponding acidic counter-ions (chloride, chlorate, bromide, bromate, perchlorate, maleate, fumarate,tartrate, succinate, phosphate, citrate, benzosulfonate, toleuensulfonate) can be separated and quantitated in the same run on reversed-phase anion-exchange column. Basic hydrophobic drugs are retained by the reversed-phase mechanism, and counter-ions are retained by the reversed-phase and anion-exchange mechanism. Some polar counter-ions are retained only by the anion-exchange mechanism. Retention time and selectivity of HPLC separation of drugs and counter-ions can be achieved by changing the amount of acetonitrile and the amount of ions in the mobile phase. The detection technique depends on the properties of the counter-ions. In case of low or no UV activity, ELSD can be employed if the counter-ion forms a non-volatile salt with the mobile phase additive (ammonium formate). This HPLC method can be used for simultaneous quantitation of other basic drugs and counter-ions. The presence of two mechanisms of retention allows control over retention times of drug and counter-ion independently, and even allows a change of order of elution when necessary.
| Column | Primesep D , 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, UV 270 |
| Class of Compounds | Ions, Hydrophilic, Hydrophobic, Base, Acids, Ionizable |
| Analyzing Compounds | Sodium Chloride, Sodium chloride, Sodium Chlorate, Sodium bromide, Sodium bromate, Perchloric Acid, Maleic Acid, Fumaric Acid, Tartaric Acid, Succinic Acid, Phosphoric Acid, Citric acid, Benzosulfonic acid, Dextromethorphan, Verapamil, Trimipramine |
Application Analytes:
Benzenesulfonic Acid
Bromide
Chlorate
Chloride
Citric Acid
Dextromethorphan
Fumaric Acid
Maleic Acid
Organic Acids
Perchlorate
Phosphoric Acid
Pyrilamine
Succinic Acid
Tartaric Acid
Verapamil
p-Toluenesulfonic Acid (PTSA)
HPLC Separation of Phosphorous and Phosphoric Acids
Application Notes: Phosphorus and phosphoric acid were separated on Obelisc N and Primesep D columns. On Obelisc N both acids retained by combination of HILIC and anion-exchange mechanisms. OnPrimesep D these inogranic acids are retained by anion-exchange mechanism. Both columns and methods can be used for analysis of hydrophilic organic and inorganic acids with multi-mode or single mode approach
Application Columns: Primesep D, Obelisc N
Application compounds: Phosphorous Acid, Phosphoric Acid
Detection technique: UV, LC/MS, ELSD/CAD
| Column | Primesep D, 3.2x50 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | AmFm |
| Flow Rate | 0.5 ml/min |
| Detection | ELCD |
| Column | Obelisc N, 4.6x50 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | AmFm |
| Flow Rate | 0.5 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid, Fungicide, Hydrophilic, Ionizable |
| Analyzing Compounds | Phosphorous Acid, Phosphoric Acids |
Application Analytes:
Phosphoric Acid
Phosphorous acid
HPLC Separation of Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column
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| Column | Newcrom B, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 20/78% |
| Buffer | Formic Acid - 2% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Acids, Hydrophilic, Ionizable |
| Analyzing Compounds | Ethylphosphonic acid, Phosphoric, Methylphosphonic Acid, Methylenediphosphonic acid |
Application Analytes:
Ethylphosphonic Acid
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate
Phosphoric Acid
HPLC Separation of Glyphosate, Glyphosine, Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column
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| Column | Newcrom B, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN Gradient -40-10%, 15 min |
| Buffer | Formic Acid Gradient -1- 5%, 15 min |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Acids, Plant growth regulator, Herbicide, Hydrophilic, Ionizable |
| Analyzing Compounds | Ethylphosphonic acid, Phosphate Ion, Methylphosphonate, Methylenediphosphonic acid, Glyphosate, Glyphosine |
Application Analytes:
Ethylphosphonic Acid
Glyphosate
Glyphosine
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate
Phosphoric Acid
HPLC Separation of Phosphoric and Phosphorous Acids on Newcrom B Column
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| Column | Newcrom B, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 20/80% |
| Buffer | Formic Acid - 2% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | Hydrophilic, Acid |
| Analyzing Compounds | Phosphoric acid, Phosphorous acid |
Application Analytes:
Phosphoric Acid
Phosphorous acid