Polyethylenimine is an amino-based hydrophilic polymer. Polyethyleneimine are used in the cell culture of weakly anchoring cells to increase attachment. Due to the fact that it is a complex mixture of polymers with different molecular weight it is very hard to obtain a single sharp peak for this compound. Polyethylenimine was analyzed on a Promix MP column. The method can be used for quantitation of polyethyleneimines in plasma, blood, saliva and other biological fluids. ELSD and LC/MS methods can be employed for detection.
Column | PEI, 4.6x250 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O - 40/60% |
Buffer | AmFM pH 3.0 - 20 mM |
Flow Rate | 0.5 ml/min |
Detection | UV, 285 nm |
Class of Compounds | Polymer, Basic, Hydrophilic, Ionizable |
Analyzing Compounds | PEI |