Polyethylenimine

Applications:


HPLC Analysis of Polyethylenimine in Plasma


Polyethylenimine is an amino-based hydrophilic polymer. Polyethyleneimine are used in the cell culture of weakly anchoring cells to increase attachment. Due to the fact that it is a complex mixture of polymers with different molecular weight it is very hard to obtain a single sharp peak for this compound. Polyethylenimine was analyzed on a Promix MP column. The method can be used for quantitation of polyethyleneimines in plasma, blood, saliva and other biological fluids. ELSD and LC/MS methods can be employed for detection.



Application Analytes:

Polyethylenimine

HPLC Determination of PEI complex with Cu(II)
HPLC Determination of PEI complex with Cu(II)_1177  

Condition

Column PEI, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 40/60%
Buffer AmFM pH 3.0  - 20 mM
Flow Rate 0.5 ml/min
Detection UV, 285 nm
 

Description

Class of Compounds Polymer, Basic, Hydrophilic, Ionizable
Analyzing Compounds PEI
 

Application Analytes:

Polyethylenimine