Polyethylenimine is an amino-based hydrophilic polymer. Polyethyleneimine are used in the cell culture of weakly anchoring cells to increase attachment. Due to the fact that it is a complex mixture of polymers with different molecular weight it is very hard to obtain a single sharp peak for this compound. Polyethylenimine was analyzed on a Promix MP column. The method can be used for quantitation of polyethyleneimines in plasma, blood, saliva and other biological fluids. ELSD and LC/MS methods can be employed for detection.
|Column||PEI, 4.6x250 mm, 5 µm, 100A|
|Mobile Phase||MeCN/H2O - 40/60%|
|Buffer||AmFM pH 3.0 - 20 mM|
|Flow Rate||0.5 ml/min|
|Detection||UV, 285 nm|
|Class of Compounds||Polymer, Basic, Hydrophilic, Ionizable|