Applications:
HPLC Analysis of Polyethylenimine in Plasma
Polyethylenimine is an amino-based hydrophilic polymer. Polyethyleneimine are used in the cell culture of weakly anchoring cells to increase attachment. Due to the fact that it is a complex mixture of polymers with different molecular weight it is very hard to obtain a single sharp peak for this compound. Polyethylenimine was analyzed on a Promix MP column. The method can be used for quantitation of polyethyleneimines in plasma, blood, saliva and other biological fluids. ELSD and LC/MS methods can be employed for detection.
Application Analytes:
Polyethylenimine PEI
HPLC Determination of PEI complex with Cu(II)
Polyethyleneimine (PEI) is a polymer used in a wide range of applications. It is difficult to analyze in HPLC due to several factors. It’s a mixture of compounds with different lengths and therefore different number of charges. It also lacks a UV chromophore. New PEI column was designed specifically for the analysis of polyethyleneimine by ion-exclusion and size-exclusion mechanisms with copper complex to allow for UV detection. The method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate buffer (AmFm) and UV detection at 285nm.
MEASURING PEI Polyethylenimine in biological samples
| Column | PEI, 4.6x250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 40/60% |
| Buffer | AmFM pH 3.0 - 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 285 nm |
| Class of Compounds | Polymer, Basic, Hydrophilic, Ionizable |
| Analyzing Compounds | PEI |
Application Analytes:
Polyethylenimine PEI