Polylysine

1

Synonyms

    Applications:

    HPLC Method for Analysis of Polylysine on BIST™ B+ Column

    November 30, 2022

    HPLC Method for Analysis of Polylysine on BIST B+ by SIELC Technologies.

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

    HPLC Method for Analysis of Polylysine on BIST™ B+ Column
    HPLC Method for Analysis of Polylysine on BIST B+ by SIELC Technologies.

    High Performance Liquid Chromatography (HPLC) Method for Analyses of Polylysine

    Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, polylysine can be retained and UV detected at 205 nm.


    Condition

    ColumnBIST B+, 4.6×50 mm, 5 µm, 100A
    Mobile PhaseGradient MeCN
    BufferH2SO4 – 0.2%
    Flow Rate1.0 ml/min
    DetectionUV 205 nm
    Peak Retention Time

    Description

    Class of CompoundsPeptide, Homopolypeptide
    Analyzing Compounds Polylysine

    Application Column

    BIST B+

    BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

    Select options
    Application Analytes:
    Polylysine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Method for Analysis of Low MW Polylysine on BIST™ B+ Column

    November 30, 2022

    HPLC Method for Analysis of Low MW Polylysine on BIST B+ by SIELC Technologies.

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

    HPLC Method for Analysis of Low MW  Polylysine on BIST™ B+ Column
    HPLC Method for Analysis of Low MW Polylysine on BIST B+ by SIELC Technologies.

    High Performance Liquid Chromatography (HPLC) Method for Analyses of Low MW Polylysine

    Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, polylysine can be retained and UV detected at 205 nm.


    Condition

    ColumnBIST B+, 4.6×50 mm, 5 µm, 100A
    Mobile PhaseGradient MeCN
    BufferH2SO4 – 0.2%
    Flow Rate1.0 ml/min
    DetectionUV 205 nm
    Peak Retention Time

    Description

    Class of CompoundsPeptide, Homopolypeptide
    Analyzing Compounds Polylysine

    Application Column

    BIST B+

    BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

    Select options
    Application Analytes:
    Polylysine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Method for Analysis of Narrow Fractions of Polylysine on BIST B+ Column

    November 30, 2022

    HPLC Method for Analysis of Narrow Fractions of Polylysine on BIST B+ by SIELC Technologies.

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

    HPLC Method for Analysis of Narrow Fractions of Polylysine on BIST B+ Column
    HPLC Method for Analysis of Narrow Fractions of Polylysine on BIST B+ by SIELC Technologies.

    High Performance Liquid Chromatography (HPLC) Method for Analyses of Narrow Fractions of Polylysine

    Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, polylysine can be retained and UV detected at 210 nm.


    Condition

    ColumnBIST B+, 4.6×50 mm, 5 µm, 100A
    Mobile PhaseGradient MeCN
    BufferH2SO4 – 0.2%
    Flow Rate1.0 ml/min
    DetectionUV 205 nm
    Peak Retention Time2.9 min

    Description

    Class of CompoundsPeptide, Homopolypeptide
    Analyzing Compounds Polylysine

    Application Column

    BIST B+

    BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

    Select options
    Application Analytes:
    Polylysine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Separation of Small Lysine-based Peptide Oligomers on BIST B+ Column

    July 8, 2011

     

    HPLC Method for Analysis of Polylysine on BIST™ B+ Column

    Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, polylysine can be retained and UV detected at 215 nm.

    Condition

    Column BIST B+, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer H3PO4
    Flow Rate 1.0 ml/min
    Detection UV 215 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Lysine-based Peptide Oligomers

     

    Application Column

    BIST B+

    Column Diameter: 4.6 mm
    Column Length: 150 mm
    Particle Size: 5 µm
    Pore Size: 100 A

    Add to cart
    Application Analytes:
    Lysine
    Polylysine

    Application Detection:
    ELSD Detection
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Analysis of Polylysine on Primesep AP Column

    October 14, 2010


    Polylysine is a small natural homo-polymer made out of amino acid lysine that is produced by bacterial fermentation. Due to the fact that it is a complex mixture of polymers with different molecular weight it is very hard to obtain a single sharp peak for this compound. Polylysine was successfully analyzed on a Primesep AP column in HILIC/cation-exclusion mode. Cation-exclusion mode on a Primesep AP column allows to obtain sharper peak. Presence of the basic groups on the surface of silica eliminated silanol interactions between basic sites of polylysine and acidic sites of Primesep AP column.

    Application Column

    Primesep AP

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Polylysine

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.