Applications:
Reverse Elution Order in Separation of Alkali Cation

Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).
Condition
Column |
Primesep C, 4.6x50 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
AmAc |
Flow Rate |
1.0 ml/min |
Detection |
ELSD 50C |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Potassium, Lithium, Chloride |
LithiumPotassiumSodium
Potassium Analysis in Organic Reaction Product

Primesep 100 separates a mixture of an organic starting material, its product, and residual potassium ion. The potassium cation is retained by cation exchange and the organic starting material and product are retained by hydrophobic and ionic interactions. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is UV, MS, and evaporative light scattering detection (ELSD) compatible.
Potassium
Complex of Cations on Primesep C is Effected by Organic Modifier

Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. In addition, the elution order can be rearranged by simply changing the organic modifier in the mobile phase from acetonitrile to methanol. The mobile phase mixture of water, acetonitrile (MeCN, ACN) or methanol (MeOH) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).
Condition
Column |
Primesep C, 4.6x50 mm, 5 µm, 100A |
Mobile Phase |
MeOH, MeCN |
Buffer |
AmAc pH 5.0 |
Flow Rate |
1.0 ml/min |
Detection |
ELSD 50C |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Potassium, Lithium, Chloride |
LithiumPotassiumSodium
Separation of Metal Ions on Primesep 100 Column

Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.
Condition
Column |
Primesep 100, 4.6x250 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
TFA |
Flow Rate |
1.0 ml/min |
Detection |
ELSD 50C |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Phosphate, Magnesium, Calcium, Lithium, Zinc |
CalciumChlorideLithiumMagnesiumPotassiumSodiumZinc
Simultaneous Separation of Cations and Anions

Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium captions by cation exchange. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD).
Condition
Column |
Primesep 100, Primesep B , 4.6x50 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
AmAc pH 5.0 |
Flow Rate |
1.0 ml/min |
Detection |
ELSD 50C |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Potassium, Bromide, Chloride |
BromideChloridePotassiumSodium
Effect of Concentration of Organic Modifier on Retention of Cation and Anions

Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium cations by cation exchange. The retention can be adjusted by changing the water/acetonitrile ratio in the mobile phase. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate with evaporative light scattering detection (ELSD).
Condition
Column |
Primesep 100, Primesep B , 4.6x50 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
AmAc pH 5.0 |
Flow Rate |
1.0 ml/min |
Detection |
ELSD |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Potassium, Bromide, Chloride |
BromideChloridePotassiumSodium
Separation of Sodium, Potassium, Lithium, and Ammonium in Mixed-Mode HPLC
Inorganic cations and ammonia are usually analyzed by ion-exchange chromatography with a conductivity detector. Mixed-mode chromatography achieves the same goal with an ELSD detector. In addition to retaining ions, mixed-mode column can retain hydrophobic molecules also, making it possible to analyze a wide variety of ionic and non-ionic compounds in one run. In this method sodium, potassium, and lithium ions were separated along with ammonium ions on a Primesep 100 column. Detection technique is ELSD.
Condition
Column |
Primesep 100, 4.6x250 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
TFA |
Flow Rate |
1.0 ml/min |
Detection |
ELSD |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Potassium, Bromide, Ammonium |
Ammonium IonLithiumPotassiumSodium
HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Obelisc N
Ion chromatography is usually used for analysis of hydrophilic organic and inorganic ions. Same separation can be achieved on HILIC/mixed-mode Obelisc N HPLC columns. Obelisc N HPLC columns have very polar groups on their surface: one of the groups is basic and the other acidic. In case of low organic concentration, two groups are connected by hydrophilic linker. Obelisc N column can be used as cation-exchange and anion-exchange column. This allows to separate positively and negatively charged molecules in one run. Five anions (chloride, bromide, methanesulfonate, nitrate and perchlorate) along with one cation (sodium) were separated in one run. Method is compatible with ELSD, CAD and LC/MS and can be used for analysis of various hydrophilic and hydrophobic cations and anions in one HPLC run.
Condition
Column |
Obelisc N, 4.6x150 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
AmFm |
Flow Rate |
1.0 ml/min |
Detection |
ELSD |
Description
Class of Compounds
|
Hydrophilic, Ions |
Analyzing Compounds |
Chloride, Nitrate, Chlorate, Bromide, Potassium |
BromideChlorideMethanesulfonic AcidNitrateNitric AcidPotassium
Simultaneous Analysis of Glucose, Sodium, Potassium and Calcium Ions
Neutral sugar, monovalent and divalent cations were separated in HILIC/cation-exchange mode on a Primesep N mixed-mode column. Mobile phase is compatible with LC/MS and method can be used for analysis polar molecules in HILIC, cation-exchange or anion-exclusion modes.
Condition
Column |
Primesep N, 4.6x150 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
AmAc pH 4.0 |
Flow Rate |
1.0 ml/min |
Detection |
ELSD 50C |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable |
Analyzing Compounds |
Potassium, Sodium, Calcium, Glucose |
CalciumPotassiumSodium
HPLC Separation of Sodium, Potassium Ions and Tetramethylammonium Chloride on Newcrom AH Column
View on hplc.cloud
Tetramethylammonium chloride is a quaternary ammonium salt used widely as a reagent in industrial applications. It can be separated from sodium and potassium chlorides on a mixed-mode Newcrom AH column with a simple isocratic MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.
Condition
Column |
Newcrom AH, 4.6x150 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O - 50/50% |
Buffer |
AmFm pH 3.0- 10 mM |
Flow Rate |
1.0 ml/min |
Detection |
CAD (Corona) MS- compatible mobile phase |
Description
Class of Compounds |
Hydrophilic, Metal, Ion |
Analyzing Compounds |
Sodium, Potassium, Tetramethylammonium |
PotassiumSodiumTetramethylammonium
Separation of Sodium and Potassium Ions on Newcrom AH Column
View on hplc.cloud
Sodium and potassium ions can be separated on a mixed-mode Newcrom AH column with a simple isocratic mobile phase of water, acetonitrile (ACN) and methanesulfonic acid. It can be detected using a conductivity detector.
Condition
Column |
Newcrom AH, 4.6x150 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O - 50/50% |
Buffer |
Methanesulfonic acid - 10 mM |
Flow Rate |
1.0 ml/min |
Detection |
Conductivity |
Description
Class of Compounds |
Hydrophilic, Metal, Ion |
Analyzing Compounds |
Sodium, Potassium |
PotassiumSodium
Separation of Silver and Potassium Ions on Newcrom AH Column
View on hplc.cloud
Silver and potassium ions can be separated on a mixed-mode Newcrom AH column with a simple isocratic mobile phase consisting of water, acetonitrile (ACN) and methanesulfonic acid. By adjusting the amount of acetonitrile used in the mobile phase, the elution order of the ions can be reversed. The ions can be detected using a conductivity detector.
Condition
Column |
Newcrom AH, 4.6x150 mm, 5 µm, 100A |
Mobile Phase |
MeCN |
Buffer |
Methanesulfonic acid - 10 mM |
Flow Rate |
1.0 ml/min |
Detection |
Conductivity |
Description
Class of Compounds |
Hydrophilic, Metal, Ion |
Analyzing Compounds |
Silver, Potassium |
PotassiumSilver