Applications:
HPLC Separation of Chlorate and Bromate Ions

Primesep B2 separates chlorate and bromate by an ion-exchange mechanism. The anion-exchange properties of the column allow retention of hydrophilic compounds, and these properties can be adjusted by simply changing the ammonium formate buffer concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and ammonium formate with evaporative light scattering detection (ELSD).
Condition
Column |
Primesep B2, 4.6x150 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O - 30/70% |
Buffer |
AmFm pH 3.0 |
Flow Rate |
1.0 ml/min |
Detection |
CAD |
Description
Class of Compounds |
Ions, Hydrophilic, Ionizable |
Analyzing Compounds |
Sodium Chlorate, Sodium Bromate |
BromateChlorateSodium BromateSodium Chlorate
HPLC Determination of Chloride, Chlorate and Perchlorate on Newcrom B Column
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Main source of contamination of environment by perchlorates are rocket fuels, car airbags, and fireworks.
EPA developed regulation of the perchlorate level in drinking water which already adapted by several states.
The most convenient, universal and very sensitive way to measure perchlorate is chromatography.
SIELC developed a simple, rugged, and selective HPLC method which allows to measure perchlorate in different matrices including drinking water.
This method allows to measure simultaneously other chloro containing ions such as chloride, and chlorate.
The method shows high selectivity and specificity.
The mobile phase is a simple mixture of water acetonitrile and ammonium formate.
The column used in the analysis has advanced surface chemistry with long chain holding a terminal positively changed functional group.
Condition
Column |
Newcrom B, 4.6x150 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O - 10/90% |
Buffer |
Gradient AmFm pH 3.0 - 40-100 mM , 10 min |
Flow Rate |
1.0 ml/min |
Detection |
CAD |
Description
Class of Compounds |
Ions, Hydrophilic, Ionizable |
Analyzing Compounds |
Sodium Chloride, Sodium Chlorate, Sodium Perchlorate |
ChlorateChloridePerchlorateSodium Chlorate