Sulfanilamide

CAS Number 63-74-1
Molecular Formula C6H8N2O2S
Molecular Weight 172.202 g/mol
InChI Key FDDDEECHVMSUSB-UHFFFAOYSA-N
LogP -0.6
Synonyms
  • sulfanilamide
  • 4-Aminobenzenesulfonamide
  • 63-74-1
  • Sulphanilamide
  • Sulfamine
  • Sulphonamide
  • p-Aminobenzenesulfonamide
  • Sulfonylamide
  • p-Aminobenzenesulfamide
  • Bacteramid
  • Streptasol
  • p-Sulfamoylaniline
  • Prontosil Album
  • Sulfonamide P
  • Estreptocida
  • Exoseptoplix
  • Streptoclase
  • p-Sulfamidoaniline
  • Streptocide
  • Sulfamidyl
  • Sulfanalone
  • Sulfanidyl
  • Sulfanil
  • Sulfocidine
  • Sulfana
  • Sulfanilimidic acid
  • p-Anilinesulfonamide
  • p-Aminophenylsulfonamide
  • Ambeside
  • Antistrept
  • Astreptine
  • Astrocid
  • Bactesid
  • Collomide
  • Colsulanyde
  • Copticide
  • Deseptyl
  • Ergaseptine
  • Erysipan
  • Gombardol
  • Lysococcine
  • Neococcyl
  • Orgaseptine
  • Prontalbin
  • Prontylin
  • Proseptal
  • Proseptine
  • Proseptol
  • Pysococcine
  • Septanilam
  • Septinal
  • Septolix
  • Septoplex
  • Septoplix
  • Strepamide
  • Strepsan
  • Streptagol
  • Streptamid
  • Streptamin
  • Streptocid
  • Streptocom
  • Strepton
  • Streptopan
  • Streptosil
  • Streptozol
  • Streptozone
  • Streptrocide
  • Sulfocidin
  • Therapol
  • 4-Sulfamoylaniline
  • Albexan
  • Albosal
  • Dipron
  • Gerison
  • Infepan
  • Sanamid
  • Stramid
  • Tolder
  • Benzenesulfonamide, 4-amino-
  • Lusil
  • 4-Aminobenzene-1-Sulfonamide
  • Prontosil White
  • Pronzin Album
  • Septamide Album
  • Stopton Album
  • Streptocid album
  • Prontosil I
  • Rubiazol A
  • White streptocide
  • PABS

Applications:


Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC



Primesep 100 separates a mixture of sulfonamides (benzenesulfonamide, chlorobenzenesulfonamide, hydroxybenzenesulfonamide) and hydroxysulphaminophenylhydrazine by a mixture of polar and hydrophobic interactions. The stationary phase’s hydrophobic functionality provides a reversed-phase mechanism while the embedded cation-exchange group provides polar interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. This method is mass spec (LC/MS) and evaporative light scattering (ELSD) compatible.



Application Analytes:

Benzenesulfonamide
Chlorobenzenesulfonamide
Hydroxybenzenesulfonamide
Hydroxysulphaminophenylhydrazine
Sulfanilamide
Sulfonamides

HPLC Separation of Aromatic Sulfonamides and Hydrozine





Application Analytes:

Benzenesulfonamide
Chlorobenzenesulfonamide
Hydroxybenzenesulfonamide
Hydroxysulphaminophenylhydrazine
Sulfanilamide

HPLC Separation of Benzenesulfonamide and Sulfanilamide
HPLC.cloud View on hplc.cloud

Condition 1

Column Newcrom А, 3.2x50 mm, 3 µm, 100A
Mobile Phase MeOH/H2O - 80/20%
Buffer H2SO4 - 0.005%
Flow Rate 0.5 ml/min
Detection  275 nm

Condition 2

Column Primesep D, , Primesep 100 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer Formic Acid - 0.1%
Flow Rate 0.5 ml/min
Detection UV 230 nm
Both Benzenesulfonamide and sulfanilamide are organic antibacterial compounds differing only in the addition of an extra amino group in sulfanilamide. The closely related compounds can be separated on a mixed-mode Primesep D column by hydrophobic and anion-exchange mechanisms or Primesep 100 column by hydrophobic and cation-exchange mechanisms with the elution order reversed using identical mobile phases. UV Detection at 230nm.

Application Analytes:

Benzenesulfonamide
Sulfanilamide

HPLC Separation of Antibiotics
HPLC.cloud View on hplc.cloud Antibiotics are widely used for treatment and prevention of bacterial infections.  Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups.  Primesep 200 has weak acidic ion-exchange pairing groups while Newcrom A has strong acidic ion-exchange groups.  In addition, methanol can be used in the mobile phase on Newcrom A column.  The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer.  UV detection at 275nm.

Condition 1

Column Primesep 200, 4.6x100 mm, 5 µm, 100A
Mobile Phase MeCN Gradient
Buffer H2SO4 - 0.5%
Flow Rate 1.0 ml/min
Detection UV 275 nm

Condition 2

Column Newcrom A, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeOH Gradient
Buffer H2SO4 Gradient
Flow Rate  0.5 ml/min
Detection UV 275 nm
 

Description

Class of Compounds Drugs, Antibiotics
Analyzing Compounds Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline


Application Analytes:

Dapsone
Sulfadiazine
Sulfaguanidine
Sulfanilamide
Sulphaquinoxaline

LinkedIn
Share
Instagram
Follow by Email