Sulfate

CAS Number 14808-79-8
Molecular Formula O4S
Molecular Weight 96.060 g/mol
InChI Key QAOWNCQODCNURD-UHFFFAOYSA-L
LogP -2.20
Synonyms
  • Sulfate
  • Sulfato(2-), ion(2-)
  • 14808-79-8
  • Sulfate (ion 2-)
  • Sulfate anion
  • Sulfate anion(2-)
  • Sulfate dianion
  • Sulfate ion
  • Sulfate ion (SO42-)
  • Sulfate(2-)
  • Sulfuric acid, ion(2-)
  • Sulphate
  • Sulfate ions
  • Sulfate radical anion
  • UNII-7IS9N8KPMG
  • SO4(2-)
  • Sulfuric acid ion(2-)
  • [SO4](2-)
  • sulphate ion

Applications:

HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC
Sulfate and phosphate are separated on Primesep B2 and Primesep D column by anion-exchange mechanism. Because both sulfate and phosphate ions are not UV-active, ELSD is used to monitor separation of both anions. Method can be used to quantitate phosphate and sulfate in various pharmaceutical and chemical formulation, products and solution, drinking and ground water. Retention time is adjusted by increase or decrease of buffer concentrations. Two anions can be retained and separated on a very short column due to strong ion-exchange interaction. Method shows good reproducibility and versatility.

Condition
Column Primesep B2, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD
 

Description
Class of Compounds Ions,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Sodium, Phosphate, Sulfate

Application Analytes:

Phosphate
Sodium
Sulfate
HPLC Analysis of Sulfate and Phosphate Ions on Primesep Columns


Inorganic and organic ions are usually analyzed by ion-exchange mechanism. In this application, we used short Primesep B2 and Primesep D mixed-mode HPLC columns to retain and separate phosphate and sulfate ions. Both ions are retained by ion-exchange mechanism. Detection technique for analysis of these anions is Evaporative Light-Scattering Detector (ELSD).


Application Analytes:

Phosphate
Sulfate
HPLC Separation of Sulfamic and Sulfuric Acid on Primesep N HILIC Column



Sulfuric acid (sulfate ions) and sulfamic acid are analyzed in HILIC mode on Primesep N column. Both compounds are hydrophilic in nature and lack UV activity. Compounds are retained by hydrophilic interaction mechanism. Sulfate ion and sulfamic acid are monitored by ELSD. Method can be used for analysis of other inorganic ions.


Application Analytes:

Amidosulfonic Acid
Aminosulfonic Acid
Sulfamic Acid
Sulfate
Simultaneous Determination of Sodium and Sulfate Ions on Obelisc N Mixed-mode HILIC Column
Inorganic cations and anions are usually analyzed by two different methods - cation-exchange and anion-exchange. This requires development and validation of two methods. Both ions can be analyzed on mixed-mode HILIC column with ELSD detection. Both ions are retained by combination of HILIC and ion-exchange mechanism.

Condition
Column Obelisc N, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm, pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD
 

Description
Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Sulfate Ions
 
Application Analytes:

Sodium
Sulfate
HPLC Separation of Inorganic Anions on Newcrom BH Column
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Condition
Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer Gradient AmAc pH 5.0 -  20-90 mM , 20 min
Flow Rate 1.0 ml/min
Detection CAD (Corona)  (MS-compatible mobile phase)

Description
Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Phosphate, Chloride, Bromide, Nitrate, Iodide, Sulfate,  Perchlorate

Application Analytes:

Bromide
Chloride
Iodide
Iodine
Nitrate
Perchlorate
Phosphate
Sodium
Sulfate