TRIS (Tromethamine)

CAS Number 77-86-1
Molecular Formula C4H11NO3
Molecular Weight 121.136 g/mol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
LogP -2.9
Synonyms
  • Trometamol
  • Tris
  • TROMETHAMINE
  • Tris(Hydroxymethyl)aminomethane
  • 77-86-1
  • Tham
  • Trisamine
  • 2-Amino-2-(hydroxymethyl)propane-1,3-diol
  • Trizma
  • Tris buffer
  • Tris base
  • 2-Amino-2-(hydroxymethyl)-1,3-propanediol
  • Trisaminol
  • Tromethane
  • Pehanorm
  • Talatrol
  • Trisamin
  • Trispuffer
  • Tutofusin tris
  • Apiroserum Tham
  • Tris-steril
  • Addex-tham
  • Tris-base
  • Tris, free base
  • Trimethylolaminomethane
  • 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-
  • Tris Amino
  • Aminotrimethylolmethane
  • Aminotris(hydroxymethyl)methane
  • THAM-E

Applications:


HPLC Separation of TRIS


TRIS or tris(hydroxymethyl)aminomethane, is a hydrophilic basic compounds widely used in chemistry, biochemistry, and molecular biology. TRIS pKa (8.1) makes it an effective buffer in pH range of 7-9.2. TRIS is not retained on reversed-phase column and is not UV active, making it hard to be analyzed by HPLC. TRIS was analyzed by mixed-mode HPLC on a Primesep 100 column. Retention time of compound is controlled by the amount and pH of the buffer. No ion-pairing reagent is required for HPLC retention of hydrophilic basic molecules like TRIS. TRIS can be monitored by ELSD/CAD with trifluoroacetic acid in the mobile phase, or by LC/MS with ammonium formate or acetate in the mobile phase.



Application Analytes:



TRIS (Tromethamine)
Trisamine