Thymine

CAS Number 65-71-4
Molecular Formula C5H6N2O2
Molecular Weight 126.115 g/mol
InChI Key RWQNBRDOKXIBIV-UHFFFAOYSA-N
LogP -0.620
Synonyms
  • Thymine
  • 5-Methylpyrimidine-2,4(1H,3H)-dione
  • 2,4(1H,3H)-Pyrimidinedione, 5-methyl-
  • 65-71-4
  • 2,4(1H,3H)-Pyrimidinedione, 5-methyl-
  • 2,4-Dihydroxy-5-methylpyrimidine
  • 4-Hydroxy-5-methylpyrimidin-2(1H)-one
  • 5-Methyl-1,2,3,4-tetrahydropyrimidine-2,4-dione
  • 5-Methyl-2,4-(1H,3H)-pyrimidinedione
  • 5-Methyl-2,4(1H,3H)-pyrimidinedione
  • 5-Methyl-2,4-dihydroxypyrimidine
  • 5-Methylpyrimidine-2,4-dione
  • 5-methyluracil
  • 5-Methyluracile
  • 5-metiluracilo
  • NSC 14705
  • NSC 168663
  • PYRIMIDINE-2,4-DIONE (1H,3H), 5-METHYL-
  • EINECS 200-616-1
  • Thymine anhydrate
  • UNII-QR26YLT7LT
  • 5-Methylpyrimidine-2,4-dione
  • 5-methylpyrimidine-2,4(1H,3H)-dione
  • Thy
  • Thymin
  • 3059-73-2
  • 691841-57-3

Applications:


Separation of Nucleic Bases
Primesep 200 separates with baseline resolution nucleic bases (uracil, thymine, cytosine, guanine, and adenine) by a combination of cation exchange and reversed phase. Uracil typically does not retain on reversed-phase column and is often used as an unretained void volume marker for C18 and C8 columns. Primesep 200 has an embedded anionic functional group which helps retain polar compounds polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.

Condition

Column Primesep 200, 4.6*250 mm,  5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer TFA - 0.2%
Flow Rate 0.5 ml/min, 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Thymine, Cytosine, Guanine, Adenine
 

Application Analytes:

Cytosine
Guanine
Nucleic Bases
Thymine
Uracil