Thymine

CAS Number 65-71-4
Molecular Formula C5H6N2O2
Molecular Weight 126.115 g/mol
InChI Key RWQNBRDOKXIBIV-UHFFFAOYSA-N
LogP -0.620
Synonyms
  • Thymine
  • 5-Methylpyrimidine-2,4(1H,3H)-dione
  • 2,4(1H,3H)-Pyrimidinedione, 5-methyl-
  • 65-71-4
  • 2,4(1H,3H)-Pyrimidinedione, 5-methyl-
  • 2,4-Dihydroxy-5-methylpyrimidine
  • 4-Hydroxy-5-methylpyrimidin-2(1H)-one
  • 5-Methyl-1,2,3,4-tetrahydropyrimidine-2,4-dione
  • 5-Methyl-2,4-(1H,3H)-pyrimidinedione
  • 5-Methyl-2,4(1H,3H)-pyrimidinedione
  • 5-Methyl-2,4-dihydroxypyrimidine
  • 5-Methylpyrimidine-2,4-dione
  • 5-methyluracil
  • 5-Methyluracile
  • 5-metiluracilo
  • NSC 14705
  • NSC 168663
  • PYRIMIDINE-2,4-DIONE (1H,3H), 5-METHYL-
  • EINECS 200-616-1
  • Thymine anhydrate
  • UNII-QR26YLT7LT
  • 5-Methylpyrimidine-2,4-dione
  • 5-methylpyrimidine-2,4(1H,3H)-dione
  • Thy
  • Thymin
  • 3059-73-2
  • 691841-57-3

Applications:


Separation of Nucleic Bases
Primesep 200 separates with baseline resolution nucleic bases (uracil, thymine, cytosine, guanine, and adenine) by a combination of cation exchange and reversed phase. Uracil typically does not retain on reversed-phase column and is often used as an unretained void volume marker for C18 and C8 columns. Primesep 200 has an embedded anionic functional group which helps retain polar compounds polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.

Condition

Column Primesep 200, 4.6*250 mm,  5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer TFA - 0.2%
Flow Rate 0.5 ml/min, 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Thymine, Cytosine, Guanine, Adenine
 

Application Analytes:

Adenine
Cytosine
Guanine
Nucleic Bases
Thymine
Uracil

HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH
HPLC.cloud View on hplc.cloud HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH Column_1245 Uracil, Thymine, Guanine, Cytosine and Adenine are the nucleobases found in RNA and DNA.  The nucleobases are difficult to separate on reverse-phase columns due to their polar, hydrophilic and ionic nature.  Using the Newcrom AH mixed-mode column, the nucleobases can be easily separated isocratically using low organic mobile phase (5% acetonitrile) or pure water, if organic mobile phase is undesirable, with ammonium formate buffer, making the method both UV and Mass Spec compatible.

Condition 1

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV 260 nm,  MS-compatible mobile phase
 

Condition 2

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase H2O - 100%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection UV 260 nm,  MS-compatible mobile phase

Description

Class of Compounds Hydrophilic, Drug, Xanthine, Nucleic Bases
Analyzing Compounds Uracil, Thymine, Guanine, Cytosine, Adenine


Application Analytes:

Adenine
Cytosine
Guanine
Thymine
Uracil