Toluidines

Applications:


HPLC Separation of Toluidine Isomers



Toluidine isomers are based on toluene and differ whether the primary amine is ortho, meta or para to the methyl group. A Primesep 200 column with an ammonium formate buffer or a Primesep 100 column with sodium phosphate baseline resolves the three regioisomers. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate, or sodium phosphate and ultraviolet (UV) detection at 250 nm.



Application Analytes:

Toluidines
m-Toluidine
o-Toluidine
p-Toluidine

HPLC Separation of Toluidine Isomers in Cation-Exchange Mode

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Isomers of toluidine were successfully retained and separated in cation-exchange mode on a Primesep S2 column. Primesep S2 column is a HILIC/cation-exchange column that can be operated in HILIC, cation-exchange, and anion-exclusion modes. Method and column are compatible with ELSD/LC/MS and UV and can be used for routine analysis of polar molecules.



Application Analytes:

Toluidines
m-Toluidine
o-Toluidine
p-Toluidine