Triton X100

CAS Number 9002-93-1
Molecular Formula C14H22O(C2H4O)n(n=9-10)
Molecular Weight 647 g/mol
Synonyms
  • polyoxyethylene octyl phenyl ether
  • Poly(oxy-1,2-ethanediyl), ?-[4-(1,1,3,3-tetramethylbutyl)phenyl]-?-hydroxy-
  • (p-t-Octylphenoxy)polyethoxyethanol
  • Anapoe X 114
  • Antarox A 200
  • ETHOXYLATED 4-TERT-OCTYLPHENOL
  • Ethoxylated octyl phenol
  • Glycols, polyethylene, mono[p-(1,1,3,3-tetramethylbutyl)phenyl] ether
  • Hydrol SW
  • Iconol OP
  • Koromex II
  • Nonidel P 40
  • Octoxynol 5
  • OCTYLPHENOXYPOLYETHYLENE
  • Ortho-Gynol
  • p-(1,1,3,3-Tetramethylbutyl)phenol ethoxylate
  • Photo-Flow 200

Applications:


HPLC Separation of Surfactants

Surfactants are molecules that contain both hydrophilic and hydrophobic groups, usually in the form of a hydrophilic head and a hydrophobic tail. Surfactants are used in detergents where they can form micelles around hydrophobic dirt molecules and wash them away. Triton X-100 is a surfactant with a hydrophilic polyethylene oxide chain that can be separated on a Primesep D reverse-phase HPLC column based on the number of oxide units in the chain. The mobile phase is water, acetonitrile (MeCN, ACN) and sulfuric acid as buffer. UV detection at 210nm.

Condition

Column Primesep D,  4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV 210nm
 

Description

Class of Compounds Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Triton X-100, Cetylpyridinium Chloride


Application Analytes:

Benzalkonium Chloride
Cetylpyridinium Chloride
Sodium
Triton X100

HILIC Separation of Surfactant Triton X-100
Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. Obelisc N HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved by hydrophilic interaction chromatography. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (ultra-violet, ELSD, LC/MS, RI, CAD, etc.)

Condition

Column Obelisc N,  4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 5.0
Flow Rate 1.0 ml/min
Detection UV 250, 230nm
 

Description

Class of Compounds Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Triton X-100


Application Analytes:

Triton X100

HPLC Separation of the Nonionic Surfactant Triton-X-100

Condition

Column Sharc 1, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH - 100/0- 50/50%, 12  min
Buffer Formic Acid 0.1% , AmFm - 0.01%
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Nonionic Surfactant, Hydrophobic, Ionizable
Analyzing Compounds Triton-X-100
 

Application Analytes:

Triton X100