Triton X100

CAS Number9002-93-1
Molecular FormulaC14H22O(C2H4O)n(n=9-10)
Molecular Weight647
Synonyms
  • polyoxyethylene octyl phenyl ether
  • Poly(oxy-1,2-ethanediyl), ?-[4-(1,1,3,3-tetramethylbutyl)phenyl]-?-hydroxy-
  • (p-t-Octylphenoxy)polyethoxyethanol
  • Anapoe X 114
  • Antarox A 200
  • ETHOXYLATED 4-TERT-OCTYLPHENOL
  • Ethoxylated octyl phenol
  • Glycols, polyethylene, mono[p-(1,1,3,3-tetramethylbutyl)phenyl] ether
  • Hydrol SW
  • Iconol OP
  • Koromex II
  • Nonidel P 40
  • Octoxynol 5
  • OCTYLPHENOXYPOLYETHYLENE
  • Ortho-Gynol
  • p-(1,1,3,3-Tetramethylbutyl)phenol ethoxylate
  • Photo-Flow 200

Applications:

HPLC Method for Separation of Hydrotopic, Cationic, Nonionic and Anion Surfactants on Newcrom BH Column

July 10, 2023

HPLC Method for Analysis of Hydrotopic, Cationic, Nonionic and Anion Surfactants on Newcrom BH by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Hydrotopic, Cationic, Nonionic and Anion Surfactants on Newcrom BH Column
HPLC Method for Separation of Hydrotopic, Cationic, Nonionic and Anion Surfactants

Surfactants, also known as surface-active agents, are compounds that lower the surface tension (or interfacial tension) between two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents, or dispersants.

They are often classified according to the charge of the polar head group:

Anionic Surfactants: These surfactants have a negative charge on their polar head group. Common examples include soap, sodium laureth sulfate, and sodium lauryl sulfate. They are commonly used in detergents and shampoos due to their ability to emulsify oils and hold dirt in suspension, so it can be rinsed away.

Cationic Surfactants: These surfactants have a positive charge on their polar head group. Examples include cetyltrimethylammonium bromide (CTAB) and benzalkonium chloride. These are often used as antiseptics and can also be found in hair conditioners because they reduce static cling.

Nonionic Surfactants: These surfactants have no charge on their polar head group. Examples include alcohol ethoxylates, nonylphenol ethoxylates, and polysorbates. Nonionic surfactants are often used in laundry and dishwasher detergents.

Hydrotropic Surfactants: While not a category of charge like the others, hydrotropic surfactants are a distinct class that promote the solubility of other solutes in water. Sodium xylene sulfonate and sodium cumene sulfonate are examples of hydrotropes.

The selection of a specific type of surfactant depends on the specific application and the properties of the surfactant. Some surfactants can be aggressive and irritating to skin or eyes (like certain anionic surfactants), while others are milder. Similarly, some are better at emulsifying oil or suspending dirt, while others might provide good foaming action or work better in hard water.

All compounds can be retained, separated, and analyzed using a reverse-phase Newcrom BH, 4.6 x 250 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzalkonium chloride, Cetylpyridinium Chloride, Triton X100, 1-Pentanesulfonic acid, 1-Hexanesulfonic acid, sodium salt, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, Sodium dodecyl sulfate, 1-Octanesulfonic acid

Condition

ColumnNewcrom BH, 4.6 x 250 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN -40-80%, 30 min
BufferAmmonium formate pH 3.0 – 20 mM
Flow Rate1.0 ml/min
DetectionELSD, 50C

Description

Class of CompoundsAliphatic sulfonic acid
Analyzing CompoundsBenzalkonium chloride, Cetylpyridinium Chloride, Triton X100, 1-Pentanesulfonic acid, 1-Hexanesulfonic acid, sodium salt, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, Sodium dodecyl sulfate, 1-Octanesulfonic acid

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
1-Decanesulfonic acid
1-Heptanesulfonic acid
1-Hexanesulfonic acid, sodium salt
1-Octanesulfonic acid
1-Pentanesulfonic acid
Benzalkonium chloride
Cetylpyridinium Chloride
Sodium dodecyl sulfate
Triton X100
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of the Nonionic Surfactant Triton-X-100

July 11, 2012


Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. SHARC 1 HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved in anhydrous conditions by hydrogen bonding using acetonitrile (ACN) and methanol (MeOH) as the mobile phase with formic acid and ammonium formate (AmFm) as buffer. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (UV, ELSD, LC/MS, RI, CAD, etc.)

Condition

Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH – 100/0- 50/50%, 12  min
Buffer Formic Acid 0.1% , AmFm – 0.01%
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Nonionic Surfactant, Hydrophobic, Ionizable
Analyzing Compounds Triton-X-100

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

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Application Analytes:
Triton X100

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HILIC Separation of Surfactant Triton X-100

August 22, 2008

Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. Obelisc N HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved by hydrophilic interaction chromatography. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (ultra-violet, ELSD, LC/MS, RI, CAD, etc.)

Condition

Column Obelisc N,  4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 5.0
Flow Rate 1.0 ml/min
Detection UV 250, 230nm

 

Description

Class of Compounds
Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Triton X-100

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Triton X100

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Surfactants

November 21, 2006


Surfactants are molecules that contain both hydrophilic and hydrophobic groups, usually in the form of a hydrophilic head and a hydrophobic tail. Surfactants are used in detergents where they can form micelles around hydrophobic dirt molecules and wash them away. Triton X-100 is a surfactant with a hydrophilic polyethylene oxide chain that can be separated on a Primesep D reverse-phase HPLC column based on the number of oxide units in the chain. The mobile phase is water, acetonitrile (MeCN, ACN) and sulfuric acid as buffer. UV detection at 210nm.

Condition

Column Primesep D,  4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV 210nm

 

Description

Class of Compounds
Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Triton X-100, Cetylpyridinium Chloride

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Benzalkonium chloride
Cetylpyridinium Chloride
Sodium
Triton X100

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.