Tween 80

CAS Number 9050-57-1
Molecular Formula C32H60O10
Molecular Weight 604.822 g/mol
InChI Key HDTIFOGXOGLRCB-MDZDMXLPSA-N
LogP 4.8
Synonyms
  • Polysorbate 80
  • Polysorbates
  • PSML
  • Sorbitan Derivatives
  • Sorbitan Monolaurate, Polyoxyethylene

Applications:


HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar


Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.

Application Analytes:

Amino Acids
Glycine
Histidine
Polysorbate
Polysorbate 20
Polysorbate 80
Sucrose
Tween 20
Tween 80

HPLC Separation of Barium Cation in the Presence of Citric Acid, Sucrose and Tween 80

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Barium ion and citric acid are analyzed in the presence of sucrose and Tween 80 on Obelisc N HILIC mixed-mode column. Both compounds are retained in ion-exchange mode and HILIC is not employed with this method. Obelisc N HPLC column has basic and acidic ion-exchange groups separated by long hydrophilic chain. Presence of that long chain allows both cation- and anion-exchange mechanisms to exist simultaneously. Obelisc N column can be used for analysis of cation and anions in HILIC/ion-exchange mode and in ion-exchange mode. Analytes can be monitored by ELSD/CAD and LC/MS.



Application Analytes:

Citric Acid
Sucrose
Tween 80