|Molecular Weight||916.112 g/mol|
Tylosine is an antibiotic used in veterinary medicine. This sugar-based amino compound is found naturally in fermentation products. Presence of sugar moieties and amino group make it impossible to analyze this compound by reversed-phase HPLC with LC/MS compatible conditions. Mixed-mode chromatography allows to overcome this obstacle, and retain and analyze this and other aminoglycosides on an Obelisc R column. This column is suitable for analysis of aminosugars with several basic groups in the molecule (streptomycine, neomycin, amikasin, arbekasin, dibekasin, framycetin, kanamycin, gentamycin, verdomycin, astromycin, hydromycin, etc.). In case of several amino groups, higher buffer concentration and lower pH (3-3.5) are required in order to facilitate elution from the column. This method does not use ion-pairing reagent, and is designed to replace traditional reversed-phase columns and ion-pairing reagent methods. In this application, Albedazole is used as internal standard to quantify aminoglycoside.
A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).
Tylosin is a macrolide antibiotic with a broad spectrum of activity against gram-negative and gram-positive organisms. It is highly polar and basic molecule with limited retention in reversed-phase chromatography. Tylosin and related impurities were analysed on Obelisc R trimodal column. Method is compatible with LC/MS and prep chromatography and can be used for HPLC analysis of tylosin in various biofluids.