Uridine

Applications:


HPLC Separation of Uridine and Uracil
Uracil is a naturally occurring pyrimidine derivative. Uracil is used for drug delivery and as a pharmaceutical. Uridine is a product of uracil and sugar ribose. Uridine is one of components of ribonucleic acid. Uracil and uridine are slightly basic in nature. Both compounds are hydrophilic and uracil is very often is used as a void marker in reversed-phase chromatography. Uracil and uridine are separated based on their polar properties on a Primesep N HILIC column. Uracil and uridine are monitored by UV.

Condition

Column Primesep N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 90/10%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 250 nm
 

Description

Class of Compounds Nucleosides,  Hydrophilic, Ionizable
Analyzing Compounds Uracil, Uridine
 

Application Analytes:

Uracil
Uridine

HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N

Nucleic bases are biological compounds found in genetic molecules (DNA, RNA). They can be separated on an Obelisc N column, which offers very polar characteristics and can be used with positively or negatively charged groups. Closely-eluted adenosine and uridine can be further separated by simply adjusting the pH of the mobile phase. Mobile phase is water and acetonitrile (MeCN, ACN) with Ammonium Acetate as buffer. UV detection at 250nm.

Condition

Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN -90%
Buffer AmAc
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Uracil, Uridine, Adenosine, Guanosine, Cytidine, Cytosine
   

Application Analytes:

Adenosine
Cytidine
Cytosine
Guanosine
Uracil
Uridine

HILIC Retention of Uridine and Uracil on Sielc's HILIC Columns
Uridine and uracil are separated on three HILIC columns from SIELC. Primesep N, Primesep S, and Obelisc N columns all have different polarity and different embedded acidic groups on the surface of silica gel: Primesep N is a normal-phase HPLC column with embedded acidic groups with a pKa of about 5. Primesep S is a normal-phase HPLC column with embedded acidic groups with a pKa of about 3. The Primesep S stationary phase retains basic compounds by cation-exchange at pH > 3. In traditional HILIC mode, a charged or neutral polar analyte interacts with a water layer on the polar stationary phase surface. On Obelisc N the charges are greatly separated and independently accessible, resulting in different selectivity compared to traditional HILIC and silica columns. Mobile phase composition changes the conformation of the long hydrophilic chain. All three columns can be used to analyze uridine and uracil by HPLC with UV, LC/MS and ELSD detection.

Condition

Column Primesep S, Primesep N, Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 95/5%
Buffer AmAc pH 4.0 - 5mM
Flow Rate 1.0 ml/min
Detection UV 270 nm
 

Description

Class of Compounds Nucleosides,  Hydrophilic, Ionizable
Analyzing Compounds Uracil, Uridine
 

Application Analytes:

Uracil
Uridine

HPLC Separation of Uracil and Uridine in HILIC Mode on Primesep S2 Column
chr_310.gif Uracil and uridine are separated based on their polarity. The mechanism of retention is pure HILIC mode. Retention time is controlled by varying the amount of ACN. Buffer concentration and pH will affect ionization and polarity of the stationary phase, and thus indirectly affect retention of neutral compounds. Column is compatible with all general detection techniques.

Condition

Column Primesep S2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 80/20%
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD, 50C UV 250 nm
 

Description

Class of Compounds Nucleosides,  Hydrophilic, Ionizable
Analyzing Compounds Uracil, Uridine
 

Application Analytes:

Uracil
Uridine

HPLC Separation of Thymidine, Uridine, Adenosine, Guanosine, and Cytidine Using the Hydrogen Bonding Method
  Application Notes: Nucleosides are glycosylamines consisting of nucleobase linked to ribose or deoxyribose sugar and are building blocks for DNA and RNA. These compounds are very polar and contain groups available for hydrogen bonding interaction. Thymidine, uridine, adenosine, guanosine and cytidine were separated using a hydrogen-bonding method. There is a strong correlation between the retention time and mobile phase composition. The strength of hydrogen-bonding interaction increases as the number of hydroxyls in the analytes increase. Additionally the rder of elution for compounds depends on the ratio of the mobile phases: acetonitrile and methanol. Our method is compatible with LC/MS and preparative chromatography. Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site. Application Compounds: Thymidine, uridine, adenosine, guanosine and cytidine

Condition

Column Sharc 1, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer AmFm, Formic acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Thymidine, Uridine, Adenosine, Guanosine, Cytidine
   

Application Analytes:

Adenosine
Cytidine
Guanosine
Thymidine
Uridine

HPLC Separation of Nucleosides and Deoxynucleosides
Th

Condition

Column Sharc 1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer AmFm, Formic acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Thymidine, Uridine, Deoxyadenosine, Adenosine, Deoxyguanosine, Guanosine, Deoxycytidine, Cytidine
 

Application Analytes:


Adenosine
Cytidine
Deoxyadenosine
Deoxycytidine
Guanosine
Thymidine
Uridine