Applications:
Separation of Zn and Mn on Primesep 100 Column

Primesep 100 separates the divalent cations, zinc and manganese, from their counter ion, chloride. The cations are retained by cation exchange and the chloride anion is eluted by ion exclusion. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is evaporative light scattering detection (ELSD) compatible.
Condition
Column |
Primesep 100, 4.6x50 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
AmAc pH 4.0 |
Flow Rate |
1.0 ml/min |
Detection |
ELSD |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Chloride, Magnesium, Zinc |
ChlorideManganeseZinc
Separation of Metal Ions on Primesep 100 Column

Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.
Condition
Column |
Primesep 100, 4.6x250 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
TFA |
Flow Rate |
1.0 ml/min |
Detection |
ELSD 50C |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Phosphate, Magnesium, Calcium, Lithium, Zinc |
CalciumChlorideLithiumMagnesiumPotassiumSodiumZinc
Zn and Na Quantitation in Drug Substances

Primesep 100 allows the quantitation of sodium and zinc cations in a drug product sample. The cations are retained by cation exchange and the drug product is retained by hydrophobic, reversed-phase mechanisms. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD) and UV detection.
Condition
Column |
Primesep 100, 4.6x250 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
TFA |
Flow Rate |
1.0 ml/min |
Detection |
ELSD 50C |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Zinc |
ChlorideSodiumZinc