HPLC Analysis of Calcium Ions in Maple Syrup

  Application Notes: maple syrup primarily consists of sucrose and other sugars, but also has small amounts of amino acids (arginine, threonine, proline), organic acids (malic and fumaric), vitamins (niacin and riboflavin) and traces of minerals (Na, K, Ca, Mn, Mg, Zn ions). One analysis performed on maple syrup is calcium ion determination. A Primesep 100 column was used in cation-exchange mode to isolate calcium ion from other components of the maple syrup. This method can be used for quantification of calcium ion by ELSD/CAD. Limit of detection depends on the type of ELSD/CAD detector used but usually reaches 20 ppm Application Columns: Primesep 100 Application compounds: calcium ions, Maple Syrup Detection technique: LC/MS, ELSD/CAD

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Maple Syrup,  Calcium
 

Application Analytes:

Calcium
Organic Acids