HPLC Separation of Inosine and Deoxyinosine

Condition

Column Sharc 1, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH - 97/3%
Buffer Formic Acid 0.1% , AmFm - 0.01%
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Nucleoside monophosphate, Hydrophilic, Ionizable
Analyzing Compounds Inosine, Deoxyinosine
 

Application Analytes:

2-Deoxyinosine
Inosine