HPLC Separation of Triclopyr

 

Triclopyr (3,5,6-Trichloro-2-pyridinyloxyacetic acid) is sold under the brand names Turflon, Weed-B-Gon, and Brush-B-Gon. Triclopyr controls broad leaf plants and is very similar to the pesticide it replaced 2,4,5-T. The EURL (European Union Reference Laboratory) included triclopyr in an analysis of acidic pesticides using QuEChERS method. Primesep 100 and Obelisc R were used to retain and separate triclopyr from impurities. Method is LC/MS compatible and developed to be used for many pesticides.

 

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Triclopyr
 

Application Analytes:

Triclopyr