Cetirizine is a member of the diphenylmethylpiperazine group of antihistamines and it contains L- and D-stereoisomers. The drug is a major metabolite of hydroxyzine, and a racemic selective H1 receptor antagonist. In general use, it treatments of hay fever, allergy symptoms (crying, itching, sneezing, etc.), and angioedema. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Cetirizine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.