HPLC Method for Analysis of Tamsulosin


Tamsulosin is chemically 5-{(2R)-2[{2-(2-ethoxyphenoxy)ethyl} amino]propyl}-2-methoxybenzene sulfonamide and a selective α1 adrenoceptor blocking agent. It is used to treat BPH (benign prostatic hyperplasia). The treatment is associated with a low rate of serum aminotransferase elevations. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Tamsulosin. The method is UV compatible and can be used as a general approach for analyzing similar compounds.


Column Primesep 200, 3.2x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%
Buffer Gradient H3PO4 - 0.05-0.3%, 5 min, 4 min hold
Flow Rate 0.5 ml/min
Detection UV, 270 nm


Class of Compounds Drug,  Acid, Hydrophilic, Ionizable,
Analyzing Compounds Tamsulosin

Application Analytes: