HPLC Analysis of Ethephon in Hydrogen-Bonding Mode

Ethephon is an important compound for the agricultural industry. It can be corrosive, but metabolizes to ethylene quickly in plants. Ethephon was retained on SHARC 1 HILIC/anion-exclusion column. Retention was controlled by adjusting methanol in mobile phase to suppress hydrogen bonding.


Column Sharc 1, 3.2x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH - 90/10% to 50/50, 6 min
Buffer Ammonia - 30 mM
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Hydrophilic, Ionizable
Analyzing Compounds Ethephon

Application Analytes: