HPLC Analysis of Trifluoroacetic and Acetic Acid on Obelisc N Mixed-Mode HPLC Column

Trifluoroacetic acid (TFA) and acetic acid (AcOH) are very commonly used in organic chemistry. They were separated using an Obelisc N column which uses HILIC/anion-exchange. The mixed-mode of Obelisc N allows for better peak shape and retention of carboxylic acids. Sulfuric acid was used to enhance polar retention by suppressing ionization of the strong acid TFA.

Application Analytes:

Acetic Acid
TFA (Trifluoroacetic Acid)