HPLC Separation of a Complex of Quinolone Antibiotics

Quinolones are a group of synthetic broad-spectrum antibiotics with a wide variety of applications in medicine and veterinary sciences. Some quinolones have been found in food products including fish and shrimp. Primesep 100 was used to separate a mixture of quinolones and achieve baseline separation. Retention was controlled by adjusting acetonitrile in the mobile phase.

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Quinolone Antibiotics, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Oxolinic Acid, Flumequine, Ciprofloxacin, Enrofloxacin, Pefloxacin
 

Application Analytes:

Ciprofloxacin
Enrofloxacin
Flumequine
Oxolinic acid
Pefloxacin