HPLC Separation of Agricultural Compounds Found in Tea

  Tea has been known to contain residue of pesticides and fungicides such as picoxystrobin and pyrimethanil. We used Primesep 200, a reversed-phase column with weakly acidic ion-pairing groups to separate picoxystrobin, pyrimethanil and atorvastatin. A 15 minute method used to demonstrate retention control when adjusting ratio of organic to aqueous in the mobile phase. Baseline retention with good resolution was achieved.    

Condition

Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 45/55%, MeCN/H2O - 50/50%
Buffer H2SO4 - 0.1 %
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Statin, Hydrophobic, Ionizable
Analyzing Compounds Atorvastatin, Picoxystrobin, Pyrimethanil, Lipitor
 

Application Analytes:

Atorvastatin
Picoxystrobin
Pyrimethanil