HPLC Separation of Fungicides in a Green Tea Matrix

  Even though teas are known for containing polyphenols such as catechins, flavonoids and theaflavins, SIELC mixed-mode columns can be used to analyze a matrix from a commercial tea and retain target compounds. In this method, green tea was spiked with 0.005mg/ml of pesticides previously found in teas. Primesep 200 was used for it’s reverse-phase and acidic ion-pairing groups. Target compounds were retained and resolved from the majority of the tea matrix peak.  

Condition

Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer H3PO4 - 0.05 %
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Statin, Hydrophobic, Ionizable
Analyzing Compounds Atorvastatin, Picoxystrobin, Pyrimethanil, Lipitor
 

Application Analytes:

Atorvastatin
Picoxystrobin
Pyrimethanil