Step 3

We have established that your compound is hydrophobic and does not have a charge within pH 1.5-7.0. Below is an example of a separation of a similar compound. This example can be used a starting condition in your method development.

Hydrophobic neutral compounds can be analysed on any Primesep column using a variety of mobile phases. Every column provides different selectivity due to different polar group structures. Each mobile phase can also change the structure of the embedded polar function group on the column and effect selectivity.