Legacy

Legacy columns

SIELC manufactures Legacy L1, Legacy L3, Legacy L7, Legacy L8, Legacy L9, Legacy L10, Legacy L11, Legacy L12, Legacy L13, Legacy L14, Legacy L16, Legacy L18, Legacy L20, Legacy L26, Legacy L28, Legacy L44, Legacy L78 and Legacy L85 columns.

As the HPLC world adapts to scientific advancements in separation technologies, old column technologies can easily become obsolete enough to warrant chromatography companies to stop producing these columns. Sometimes, companies will shut down only that production line, and other times, companies will shutter completely as they are unable to evolve quickly enough with the ever-changing landscape.

Consumers that once depended on these out-of-production columns are now stuck – they can either overhaul entire methods and systems, or they can abandon their projects altogether. With SIELC’s Legacy column series, consumers don’t have to do either.

SIELC’s Legacy column series is based on United States Pharmacopeia’s (USP) published chromatographic methods and procedures. A plethora of brands have columns used in USP reference standards and methods, and USP has created different designations to group together columns with similar types of packing and properties in the solid phase. ple replacement. For example, L1 is one of the most popular designations, referring to columns with octadecyl silane (C18) chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter. An L1 column from one manufacturer can be swapped for another L1 column from a different manufacturer with little to no modification of the method. However, not all L1 columns are exactly the same, so relative compound retention and peak shape can be different from one brand’s column to another.

For companies that have developed integral methods based on USP columns that are no longer in production, the Legacy column series offers them a simple and easy way to find a compatible replacement.

If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here

If you would like to purchase these columns please contact us at sales@sielc.com

SIELC ColumnUSP Specification
Legacy L1Octadecyl silane chemically bonded to porous silica or ceramic particles (3 to 10µm)
Legacy L3Porous silica microparticles 1.5 to 10µm in diameter
Legacy L7Octyl silane (C8) chemically bonded to porous silica particle- 3 to 10µm in diameter
Legacy L8An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 3-10µm diameter
Legacy L9Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10 µm in diameter.
Legacy L10Nitrile groups chemically bonded to porous silica particles 3 to 10µm in diameter
Legacy L11Phenyl groups chemically bonded to porous silica particles 3 to 10µm in diameter
Legacy L12A strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 3 to 50 µm in diameter
Legacy L13Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameter
Legacy L14Silica gel, 5 to 10µm in diameter, having a chemically bonded, strongly basic quaternary ammonium anion exchanger (SAX) coating.
Legacy L16Dimethylsilane (C2) chemically bonded to totally porous silica particles- 5 to 10µm.
Legacy L18Amino and cyano groups chemically bonded to porous silica particles, 5 to 10µm in diameter
Legacy L20Dihydroxypropane (diol) groups chemically bonded to porous silica or hybrid particles, 3 to 10µm in diameter.
Legacy L26Butyl silane (C4) chemically bonded to porous silica particle- 3 to 10µm.
Legacy L28A multifunctional support which consists of a high purity, 100A, spherical silica substrate that has been bonded with anionic (amine) functionality in addition to conventional reversed-phase C8 functionality.
Legacy L44A multifunctional support, which consists of high purity, 100A, spherical silica substrate that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to a convention reversed-phase C8 functionality
Legacy L78A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and anion-exchange (primary, secondary, or tertiary amino groups) functional groups chemically bonded to porous or non-porous or ceramic micro-particles, 3 to 50µm in diameter or a monolithic rod
Legacy L85A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and weak cation-exchange (carboxyl groups) functional groups chemically bonded to porous or non-porous particles, 3 to 50µm in diameter

If you would like to purchase these columns please contact us at sales@sielc.com

Development of new Methods

While USP has produced a list of validated methods, many people alter USP methods to better suit their needs. Many of the methods listed in USP are outdated because they were developed on older instruments with outdated column technologies. Therefore, they might need to be modified to better suit new instrumentation and columns. Altering the validated USP methods can save times and improved results. 

Legacy Products