Primesep 100 is a reverse-phase analytical column with embedded acidic ion-pairing groups
This is the most versatile column of the entire Primesep family for separation of a broad range of compounds.
- Improves retention of basic compounds by cation-exchange mechanism.
- Separates acids by ion-exclusion mechanism.
- Retains neutral compounds by reverse-phase mechanism.
- All mobile phases are LC-MS and preparative chromatography compatible.
Primesep 100 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 µm, 5 µm, 3 µm, 2.7 µm (solid core)
Pores: 100 A
Primesep 100 Application Examples
HPLC Separation of β‑Hydroxy-β-methylbutyric Acid And Leucine
HPLC Determination of Ritodrine
HPLC Determination of Folic Acid
HPLC Method for Analysis of Gabapentin Tablets
HPLC Method for Analysis of Thymol Blue
Separation of allo-D-isoleucine and L- Isoleucine on Primesep 100 HPLC column
2,4-D Retention on Primesep 100 and Obelisc R
1-Naphthaleneacetic acid (NAA) Separation on Primesep and Obelisc Mixed-Mode Columns
16a-Hydroxyestradiol Separation on Mixed-Mode HPLC Columns
4-CPA Analysis on Obelisc and Primesep Mixed-Mode Columns
Amino Acids Analysis in Acid Gradient Condition
Amino Sugars Analysis in Ion Exchange and Reverse Mode
Analysis of Folic Acid and Related Impurities
Analysis of Nicotinic Acid in Nicotinamide
Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds
Controlling Retention of 2,4-DB on Mixed-Mode HPLC Columns
Controlling Retention of Azinphos-ethyl on Primesep and Obelisc HPLC Columns
Dewetting Study in Aqueous Conditions
Dichlorprop (2,4-DP) Analysis on Primesep and Obelisc HPLC Columns
Diclobutrazol Separation on Primesep and Obelisc HPLC Columns
Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds
Effect of Concentration of Organic Modifier on Retention of Cation and Anions
Folpet Analysis on Primesep 100 and Obelisc R HPLC Columns
HPLC Analysis of 17a-Ethynylestradiol
HPLC Analysis of 2,4,5-T on Mixed-Mode Columns
HPLC Analysis of 5-Fluorouracil on Primesep Columns with LC/MS Compatible Mobile Phase
HPLC Analysis of Active Drug and Amino Acids in a Formulation
HPLC Analysis of Bentazon on Primesep and Obelisc Mixed-Mode Columns
HPLC Analysis of Calcium Ions in Maple Syrup
HPLC Analysis of Cheese Extract using Mixed-Mode Chromatography
HPLC Analysis of Citrulline and Arginine on Primesep Columns
HPLC Analysis of Dichloran on Mixed-Mode Columns
HPLC Analysis of Diphenamid on Primesep 100 and Obelisc R
HPLC Analysis of Fatty Acids with MS Compatible Conditions
HPLC Analysis of Fentin on Obelisc and Primesep Mixed-Mode Columns
HPLC Analysis of Inosine on Primesep 100 Column
HPLC Analysis of MCPA on Primesep and Obelisc Mixed-Mode Columns
HPLC Analysis of Motrin Cough & Cold Composition
HPLC Analysis of Piperazine on Primesep Columns
HPLC Application for Analysis of Sodium Succinate on Primesep 100
HPLC Application for Retention of Guanidine by Mixed-Mode Chromatography
HPLC Diazinon Analysis on Primesep 100 and Obelisc R columns
HPLC Method for Analysis of Fluconazole
HPLC Method for Determination of Lysine in Ibuprofen Lysine Composition
HPLC Retention Control of Antibiotics in Toothpaste
HPLC Retention of Aminopyrazole
HPLC Retention of Chlorpyrifos-methyl on Primesep 100 and Obelisc R Mixed-Mode Columns
HPLC Retention of Copper Ion on Primesep 100 Mixed-Mode Cation-Exchange Columns
HPLC Retention of Pirimicarb on Primesep 100 and Obelisc R
HPLC Separation of 2-Naphthoxyacetic acid
HPLC Separation of 8 Generic Compounds on Primesep Columns
HPLC Separation of a Complex of Quinolone Antibiotics
HPLC Separation of Albuterol and Amoxicillin
HPLC Separation of Amino Acids
HPLC Separation of Amino Acids in Supplements Capsules
HPLC Separation of Amino Acids in Supplements Composition in Mixed-Mode
HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase
HPLC Separation of Aromatic Sulfonamides and Hydrozine
HPLC Separation of Benzenesulfonamide and Sulfanilamide
HPLC Separation of Caffeine and Phenylephrine
HPLC Separation of Captan on Obelisc R and Primesep 100 Columns
HPLC Separation of Carbofuran on Obelisc R and Primesep 100 Columns
HPLC Separation of Carboxylic Acids
HPLC Separation of Catecholamines on Primesep 100 Column with Phosphate Buffers
HPLC Separation of Cidofovirs Mixture
HPLC Separation of Codeine, Oxycodone, and Hydrocodone
HPLC Separation of Components of Excedrin
HPLC Separation of DMSO and Glycine by Mixed-Mode Chromatography
HPLC Separation of DOPAC, Homovanillic Acid and Dopamine on the Primesep 100 Column
HPLC Separation of Epinephrine and Epinephrine Sulfonate
HPLC Separation of Ethanolamines
HPLC Separation of GABA and GLU
HPLC Separation of Glutamic Acid and GABA
HPLC Separation of Glyphosate Production Intermediates
HPLC Separation of Guaifenesin and Terbutaline
HPLC Separation of Guanidine and Benzoic Acids on Primesep 100 Column
HPLC Separation of Hydrocortisone and Hydrocortisone’s Degradation Products
HPLC Separation of Impurities of Aminosugar
HPLC Separation of Isomers of Amino Salicylic Acid
HPLC Separation of Isomers of Aminobenzoic Acid
HPLC Separation of Metribuzin on Mixed-Mode HPLC Columns
HPLC Separation of Nabam on Mixed-Mode Columns
HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column
HPLC Separation of Phenylephrine with Tannic Acid Formulation
HPLC Separation of Phosphamidon on Mixed-Mode Columns
HPLC Separation of Phthalic Acids using Hydrogen Bonding
HPLC Separation of Pyridinecarboxylic Acids
HPLC Separation of Serine, a-Methylserine, GABA, and Methionine Mixture
HPLC Separation of Toluidine Isomers
Hydrophobic and Hydrophilic Compound Separation
Loading Study for Preparative Chromatography
MCPB Analysis Using Obelisc and Primesep Analytical HPLC Columns
Mecoprop Analysis on Primesep and Obelisc Mixed-Mode HPLC Columns
Nickel Contamination in Hydrogenation Products
Potassium Analysis in Organic Reaction Product
Retaining the Fungicide Carboxin on Primesep and Obelisc HPLC Columns
Retention Control of Bromacil on Primesep and Obelisc Mixed-Mode Columns
Retention of Aminolevulinic Acid in Mixed-Mode System
Retention of Monocrotophos on Primesep 100 and Obelisc R Columns
Retention of the Herbicide Dicamba on Mixed-Mode HPLC Columns
Reverse Elution Order in Separation of Alkali Cation
Reverse Mode vs Normal Mode: Separation of Aromatic Compounds
Separating Triadimefon from Impurities on Primesep and Obelisc HPLC Columns
Separation of Diacid: Ion Exclusion mode
Separation of Glyphosate by Cation Exchange and Anion Exchange
Separation of Isomers of Aminobenzoic Acid
Separation of Metal Ions on Primesep 100 Column
Separation of Oxytetracycline and Chlortetracycline in Mixed-Mode
Separation of Sodium, Potassium, Lithium, and Ammonium in Mixed-Mode HPLC
Separation of Steroids in Reverse and Normal Mode on Primesep 100 Column
Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC
Separation of Sulphonium Ions in Polar Organic Mode
Separation of Thiabendazole on Mixed-Mode HPLC Columns
Separation of Zn and Mn on Primesep 100 Column
Simultaneous Separation of Cations and Anions