Retention of the Herbicide Dicamba on Mixed-Mode HPLC Columns

  Dicamba is an organochloride derivative of benzoic acid. It is used as a non-specific herbicide that is usually used on non-crop  plants. Dicamba is moderately toxic to humans, and both persistent and mobile in soils. The EURL (European Union Reference Laboratory) tested dicamba in an analysis of acidic pesticides. Primesep 100 and Obelisc R were used to separate dicamba with unique separation. Method is LC/MS compatible

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min, 7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min, 7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min, 7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min, 7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Hydrophobic, Ionizable
Analyzing Compounds Dicamba
 

Application Analytes:

Dicamba