Separation of Benzoic and Acetylbenzoic acid in Hydrogen-Bonding Mode

A general approach for analysis of various organic acids with MS detection in negative mode was developed using hydrogen-bonding stationary phase - SHARC 1. A highly sensitive method allows to analyze traces of organic acids in various matrices using ACN/MeOH/ammonia mobile phase. In hydrogen-bonding chromatography acetonitrile is a weaker solvent and alcohol is a stronger solvent. Various gradient and isocratic conditions can be used

Application Analytes:

4-Acetylbenzoic Acid
Benzoic Acid