Ion-chromatography (IC) is the established technique for analysis of inorganic and some organic ions. However, there are some problems associated with this technology.
IC typically requires specific and expensive equipment which is not readily available in every analytical lab.
IC is a very sensitive technology, and in analysis where the concentration of analyzed ions is significant, it requires several dilution steps to bring the sample to a convenient concentration.
IC works perfectly in pure aqueous media; however, the IC instrumentation cannot be used with a significant amount of organic component in the mobile phase. This causes difficulties when an organic sample with a small amount of analyte is introduced in the IC column. Usually, it leads to contamination and destruction of the column, or requires some additional cleanup steps.
Non-charged analytes are usually not detectable or not separable within IC conditions.
Different sets of conditions are required for separation of anions and cations, and they cannot be analyzed simultaneously.
SIELC Technologies offers an alternative technology that can address the above problems.
The combination of Primesep mixed-mode stationary phase with the evaporative light scattering detecting (ELSD) technique allows you to use a broad range of mobile phases including the combination of water with acetonitrile, or methanol with acidic modifiers such as TFA, acetic and formic acid, ammonium acetate, ammonium formate, or triethylamine acetate. Using this wide variety of mobile phases, we can achieve various separations. Also, a great number of compounds can be separated using the standard HPLC equipment with the addition of ELSD only. Charged and neutral organic and inorganic analytes can be simultaneously analyzed within a single HPLC run. Direct injection of the sample without any cleanup or/and dilution is achievable.