The cell internal environment is very different from the external environment (mobile phase);
The pore walls form the dimensions and structure of the liquid separation cell;
The cell internal environment has stationary (bound) and exchangeable (mobile) components;
The pH and ionic strength inside the cell differs from outside the cell;
The pore opening acts as a membrane channel for the analytes;
The cell responds to changes in external environment with physical and chemical changes
These changes include conformational changes in the long hydrophobic and hydrophilic chains, degree of ionization, charge distribution and counter-ion properties.
