Promix Technology

Applications:

Proteomics
Peptide/Protein Analysis
Peptide/Protein Purification

Features & Benefits:

2-D HPLC with a Single Column
Ionic Interactions
Reversed-Phase
Separate peptides and proteins that differ only by a single amino acid
Enhanced selectivity for closely related peptides and proteins.
Increased peak capacity compared to reversed-phase and ion exchange.
Increased LC-MS sensitivity using formic acid and ammonium acetate.
Completely Scalable from capillary to prep

Promix™ Technology

Independent control of acid/buffer concentration and organic modifier
offers almost limitless control of retention and selectivity!

1. ARG-VAL-TYR-VAL-HIS-PRO-ILE
2. ARG-VAL-TYR-VAL-HIS-PRO-PHE
3. ALA-PRO-GLY-ASP-ARG-ILE-TYR-VAL-HIS-PRO-PHE
4. ASP-ARG-VAL-TYR-VAL-HIS-PRO-PHE-HIS LEU
5. ASP-ARG-VAL-TYR-ILE-HIS-PRO-PHE-HIS-LEU

Upper Column: Promix TM MP, 4.6 x 250 mm 300 Å Mobile phase: MeCN – gradient 15-35 % in 15 min Gradient TFA – 0.4-0.05% in 15 min;

Lower Column: Promix TM MP, 4.6 x 150 mm 300 Å Mobile phase: MeCN – gradient 0-40 % in 30 min
Gradient TFA – 0.4-0.05% in 30 min;

	1. Ribonuclease A (mw 13,500) 2. Insulin (mw 5,700) 3. Lysozyme (mw 14,300) 4. B-Lactalbumin (mw 14,200) 5. Carbonic Anhydrase (mw 29,000)