The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep B2 mixed-mode column. Primesep B2 is a reverse-phase column with embedded basic ion-pairing groups.

The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep SB mixed-mode column. Primesep SB is a reverse-phase column with embedded basic ion-pairing groups.  

Condition

Column Primesep SB, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer Gradient AmFm pH 3.0- 2-50 mM, 3 min, 3 min hold
Flow Rate 1.0 ml/min
Detection CAD
 

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Glucuronic acid, Methanesulfonic acid, Butanesulfonic acid
 

Application Analytes:

Benzenesulfonic Acid
Butanesulfonic acid
Glucuronic acid
Methanesulfonic Acid