HPLC Separation of Homovanillic Acid, Dopamine, and DOPAC using Hydrogen Bonding

Condition

Column Sharc 1, 3.2x50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH - 100/0 - 30/70%
Buffer Formic Acid - 0.5% AmFm 0.05% 7 min
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds DOPAC, Homovanillic Acid, Dopamine
 

Application Analytes:

DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Homovanillic Acid