USP Compatible Legacy “L” Columns

Many pharmaceutical companies use chromatography to analyze their products. Chromatography is easy to perform, and provides accurate, repeatable data. However, to determine repeatability, U.S. Pharmacopeia developed a validation process. USP validation for HPLC methods is detailed in the “Analytical Performance Parameters” (USP {225}). The typical analytical characteristics used in method validation include: accuracy, precision, specificity, detection limit, quantitation limit, linearity, range, and ruggedness. SIELC has developed the Legacy column, which can be used in many USP Validated methods. 

Sielc Column USP Specification
Legacy L1

 

Octadecyl silane chemically bonded to porous silica or ceramic particles (1.5 to 10µm)

 

Legacy L3 Porous silica microparticals 1.5 to 10µm in diameter
Legacy L7 Octyl silane (C8) chemically bonded to porous silica particle- 1.5 to 10µm in diameter
Legacy L8 An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 3-10µm diameter

 

Legacy L10 Nitrile groups chemically bonded to porous silica particles 3 to 10µm in diameter

 

Legacy L11 Phenyl groups chemically bonded to porous silica particles 1.5 to 10µm in diameter

 

Legacy L14 Silica gel, 5 to 10µm in diameter, having a chemically bonded, strongly basic quaternary ammonium anion exchanger (SAX) coating.

 

Legacy L16 Dimethylsilane (C2) chemically bonded to totally porous silica particles- 5 to 10µm.
Legacy L26 Butyl silane (C4) chemically bonded to porous silica particle- 3 to 10µm.
Legacy L28 A multifunctional support which consists of a high purity, 100A, spherical silica substrate that has been bonded with anionic (amine) functionality in addition to conventional reversed-phase C8 functionality.
Legacy L44 A multifunctional support, which consists of high purity, 60A, spherical silica substrate that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to a convention reversed-phase C8 functionality