USP Methods for the Separation of Propranolol and Procainamide using a Legacy L1 Column

  Application Notes: Propranolol is a common beta-blocker used for treating anxiety and hypertension, and procainamide is an antiarrhythmic drug. According to the USP methods, propranolol hydrochloride contain no less than 98% and no more than 101.5 percent of propranolol calculated on a dried basis. The USP HPLC method for the separation of phenylephrine and epinephrine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP. Application Columns: Legacy L1 C18 HPLC column Application compounds: Propranolol and procainamide Mobile phase: Water/MeOH/MeCN (70/70/90) with 7mM sodium lauryl sulfate and 11 mM phosphoric acid Detection technique: UV Reference: USP35: NF30

Condition

Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase Water/MeOH/MeCN (70/70/90) with 7mM Sodium Lauryl   sulfate and 11   mM Phosphoric acid
Buffer Sodium Lauryl   Sulfate, Phosphoric acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Beta blocker, Antiarrhythmic, Hydrophobic, Ionizable, Neutral
Analyzing Compounds Propranolol, Procainamide
 

Application Analytes:

Procainamide
Propranolol