Acrylic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Acrylic acid in combination with other monomers produces copolymers which are used in manufacturing of various products such as paints, adhesives and plastics. Esters and salts of acrylic acid are known as acrylates. 4-methoxyphenol, also known as mequinol, is an active ingredient in topical drugs and is also used for inhibition of radical polymerization of monomers like acrylates. The two compounds can be separated on a mixed-mode Primesep B column with isocratic method in mobile phase of 20/80 Acetonitrile (ACN) and water with 0.02% phosphoric acid buffer. UV Detection 200nm.