Amoxicillin hydrate (1:3)

CAS Number 61336-70-7
Molecular Formula C16H25N3O8S
Molecular Weight 419.450 g/mol
LogP 0.601
  • Amoxicillin hydrate (1:3)
  • (2S,5R,6R)-6-{[(2R)-2-Amino-2-(4-hydroxyphenyl)acetyl]amino}-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid--water (1/3)
  • 4-Thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid, 6-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino]-3,3-dimethyl-7-oxo-, (2S,5R,6R)-, hydrate (1:3)
  • 61336-70-7
  • Alfoxil
  • alpha-Amino-p-hydroxybenzylpenicillin trihydrate
  • Amoxicillin trihydrate
  • Amoksicillin forte
  • Amoxi-wolff
  • Amoxicillin-ratiopharm
  • Benzoral
  • Dura AX
  • Uro-clamoxyl
  • Paradroxil
  • Metifarma
  • Kentrocyllin
  • Amophar
  • Amoxi-Diolan
  • Amoxillat
  • Amoxina
  • Amoxine
  • Flemoxine
  • Galenamox
  • Himinomax
  • Pacetocin
  • Siganopen
  • Sintopen
  • Zamocilline
  • UNII-804826J2HU


Separation of Amoxicillin hydrate (1:3) on Newcrom R1 HPLC column

Amoxicillin hydrate (1:3) can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Separation of Amoxicillin hydrate (1:3) on Newcrom C18 HPLC column
Newcrom R1

This column is available in new type of hardware with only one connection end. Learn more about performance and advantage of this new hardware.

Application Analytes:

Amoxicillin hydrate (1:3)

HPLC Separation of β-Lactam Antibiotics on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode View on

High Performance Liquid Chromatography (HPLC) Method for Analysis of Amoxicillin and Cephalexin. Both amoxicillin and cephalexin are beta-lactam antibiotics. Beta-lactam antibiotics contain a beta-lactam ring in their molecular structure. As a group, these drugs are active against many gram-positive, gram-negative and anaerobic organisms. Both compounds can be retained and baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a Sulfuric acid (H2SO4) buffer. UV detection at 250 nm.


Column Primesep 100, 4.6x100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA, H2SO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds Drug, Antibiotics, Hydrophobic, Ionizable
Analyzing Compounds Cephalexin,  Amoxicillin

Application Analytes:

Amoxicillin hydrate (1:3)