Ampicillin trihydrate

Ampicillin trihydrate structural formula

CAS Number7177-48-2
Molecular FormulaC16H25N3O7S
Molecular Weight403.451
InChI KeyRXDALBZNGVATNY-CWLIKTDRSA-N
LogP1.12
Synonyms
  • Ampicillin trihydrate
  • (2S,5R,6R)-6-{[(2R)-2-Amino-2-phenylacetyl]amino}-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid--water (1/3)
  • 4-Thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid, 6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-, (2S,5R,6R)-, hydrate (1:3)
  • 7177-48-2
  • Amperil
  • Ampinova
  • NCI-C56086
  • Polycillin
  • Trafarbiot
  • (D-(-)-alpha-Aminobenzyl)penicillin trihydrate
  • alpha-Aminobenzylpenicillin trihydrate
  • Ampicillin 3-wasser
  • UNII-HXQ6A1N7R6
  • (2S,5R,6R)-6-{[(2R)-2-amino-2-phenylacetyl]amino}-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid trihydrate
  • aminobenzylpenicillin trihydrate

Applications:

Separation of Ampicillin trihydrate on Newcrom R1 HPLC column

May 16, 2018
Separation of Ampicillin trihydrate on Newcrom R1 HPLC column

Ampicillin is an antibiotic used to treat a number of bacterial infections. It is in the penicillin group of antibiotics and is so safe, that it is allowed for use during pregnancy. Ampicillin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Ampicillin trihydrate
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.

HPLC Method for Analysis of Principen (Ampicillin)

May 10, 2016

56

 

Condition
Column Primesep 200, 3.2×100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 30/65%
Buffer Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold
Flow Rate 0.5 ml/min
Detection UV, 270 nm

 

Description
Class of Compounds
 Drug, Penicillin antibiotic, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Principen, Ampicillin

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Ampicillin trihydrate
Principen
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.