| CAS Number | 62-53-3 |
|---|---|
| Molecular Formula | C6H7N |
| Molecular Weight | 93.129 |
| InChI Key | PAYRUJLWNCNPSJ-UHFFFAOYSA-N |
| LogP | 0.9 |
| Synonyms |
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Applications:
Separation of Aniline on Newcrom R1 HPLC column
May 16, 2018
Aniline is a compound made up of a benzene ring attached to an amino group. It is a precursor to many chemical compounds and used in rubber processing agents, dyes, and herbicides. Aniline can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsHPLC Method for Analysis of Pesticides: Thiamphenicol, Aniline, Simazine, Alachlor, Thiabendazole, Diazinon, Trifluralin
July 11, 2017
| Column | Primesep 100, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 35-85%, 6 min , 4 min hold |
| Buffer | Gradient H2SO4 – 0.05- 0.15%, 6 min, 4 min hold |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 230 nm |
| Column | Primesep B2, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 35-85%, 6 min , 4 min hold |
| Buffer | Gradient H2SO4 – 0.05- 0.15%, 6 min, 4 min hold |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Thiamphenicol, Aniline, Simazine, Alachlor, Thiabendazole, Diazinon, Trifluralin |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep B2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsAniline
Diazinon
Simazine
Thiabendazole
Thiamphenicol
Trifluralin
