Arbidol (Umifenovir)

 

CAS Number131707-25-0
Molecular FormulaC22H25BrN2O3S
Molecular Weight447.4
InChI KeyKCFYEAOKVJSACF-UHFFFAOYSA-N
LogP4.4
Synonyms
  • Arbidol
  • umifenovir
  • 131707-25-0
  • UNII-93M09WW4RU
  • Umifenovir [INN]
  • ethyl 6-bromo-4-[(dimethylamino)methyl]-5-hydroxy-1-methyl-2-[(phenylsulfanyl)methyl]-1H-indole-3-carboxylate
  • MLS000777586
  • 93M09WW4RU
  • Umifenovir (INN)
  • SMR000413980
  • ethyl 6-bromo-4-[(dimethylamino)methyl]-5-hydroxy-1-methyl-2-(phenylsulfanylmethyl)indole-3-carboxylate
  • 1H-Indole-3-carboxylic acid, 6-bromo-4-((dimethylamino)methyl)-5-hydroxy-1-methyl-2-((phenylthio)methyl)-, ethyl ester
  • Arbidol base

Applications:

HPLC-MS Method for Analysis of Arbidol (Umifenovir) on Primesep 100 Column

August 22, 2023

High Performance Liquid Chromatography (HPLC) Method for Analysis of Arbidol (Umifenovir) on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Arbidol (Umifenovir) on Primesep 100 Column by SIELC Technologies

Umifenovir, commonly known by its trade name Arbidol, is an antiviral drug. Originally developed in Russia, it has been used primarily in Russia and China for the prophylaxis and treatment of influenza and other respiratory viral infections.

Mechanism of Action:
Umifenovir interferes with the viral ability to penetrate into host cells. It acts by inhibiting the fusion of the viral lipid membrane with the host cell membrane, thus preventing the virus from entering and infecting the cell.

Uses:

  • Influenza: Umifenovir has been prescribed for influenza A and influenza B treatment.
  • Other Viral Infections: Besides influenza, the drug has also been indicated for prophylaxis and treatment of acute respiratory viral infections (ARVI) and recurrent respiratory infections.
  • Potential Role in COVID-19: In the wake of the COVID-19 pandemic, Umifenovir gained attention as a potential treatment for the virus. Some initial studies and clinical trials have been conducted to assess its efficacy against SARS-CoV-2. While it has been used in some countries as a part of COVID-19 treatment protocols, its efficacy specifically against COVID-19 is not firmly established, and research is ongoing.

Side Effects:
Though generally considered to be safe, some reported side effects of Umifenovir include digestive disturbances, allergic reactions, or elevated heart rate. However, side effects are generally rare. As always, any medication’s potential side effects should be discussed with a healthcare provider.

Availability:
While Umifenovir is available in countries like Russia and China, its acceptance and usage differ worldwide. For instance, it hasn’t been approved for antiviral use in the United States.

Arbidol can be retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Ammonium formate as a buffer. This analysis method can be detected using UV at 254 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)

High Performance Liquid Chromatography (HPLC) Method for Analyses of Arbidol (Umifenovir)

Condition

ColumnPrimesep 100, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 75/25%
BufferAmmonium Formate pH 3.0- 50 mM
Flow Rate0.2 ml/min
DetectionUV 254 nm, LCMS SIM 479+
Peak Retention Time8.17 min

Description

Class of CompoundsDrug
Analyzing CompoundsArbidol (Umifenovir)

Application Column

Primesep 100

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Arbidol (Umifenovir)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Arbidol (Umifenovir) on Newcrom AH Column

April 1, 2020


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Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 60/40%
Buffer AmFm pH 3.0- 50 mM
Flow Rate 1.0 ml/min
Detection UV 255 nm,  MS-compatible mobile phase

Description

Class of Compounds Hydrophobic, Drug
Analyzing Compounds Arbidol (Umifenovir)

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Arbidol (Umifenovir)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.