| CAS Number | 51-55-8 |
|---|---|
| Molecular Formula | C17H23NO3 |
| Molecular Weight | 289.375 |
| InChI Key | RKUNBYITZUJHSG-SPUOUPEWSA-N |
| LogP | 1.83 |
| Synonyms |
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Applications:
HPLC Separation of Mixture of Atropine, Atrolactic Acid and Pralidoxime Chloride
April 15, 2019
HPLC Method for Atropine, Atrolactic Acid, Pralidoxime Chloride on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Atropine, Atrolactic Acid, Pralidoxime Chloride.
Atropine is a tropane alkaloid with the chemical formula C17H23NO3. It is an anticholinergic medication that is used to treat nerve agent, pesticide poisoning, slow heartrate, and is also used to decrease saliva production. Typically, it is administered through injection.
Atrolactic Acid is a 2-hydroxy monocarboxylic acid with the chemical formula C9H10O3. It can cause irritation to eyes, skin, and the respiratory system.
Pralidoxime Chloride, also known as 2-PAM, is a cholinesterase reactivator with the chemical formula C7H9N2O+. It is used as an antidote for poisoning by organophosphate pesticides and nerve agents.
Atropine, Atrolactic Acid, Pralidoxime Chloride can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Gradient H3PO4 – 0.1-0.3%, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug, Acid, Base, Hydrophilic, Hydrophobic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Atropine, Atrolactic Acid, Pralidoxime Chloride |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Atropine
Pralidoxime Chloride
HPLC Determination of Atropine on Primesep 200 Column
April 5, 2019
HPLC Method for Atropine on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Atropine.
Atropine is a tropane alkaloid with the chemical formula C17H23NO3. It is an anticholinergic medication that is used to treat nerve agent, pesticide poisoning, slow heartrate, and is also used to decrease saliva production. Typically, it is administered through injection.
Atropine can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.1 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug, Basic, Hydrophobic, Ionizable, Zwitterionic |
| Analyzing Compounds | Atropine |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Separation of Atropine on Newcrom R1 HPLC column
February 16, 2018
Atropine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options